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UMASS Medical School

7 ARTICLES PUBLISHED IN JoVE

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Neuroscience

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
Timothy P. Murphy 1,2, Dan D. Luu 1, Jessica A. DeSimone 1,3, Thomas C. O'Brien 1,4, Christopher J. Lally 1,5, Jillian J. Lindblad 1,6, Sarah M. Webster 1
1Department of Biology, College of the Holy Cross, 2School of Medicine, Georgetown University, 3Department of Biochemistry, Giesel School of Medicine, Dartmouth College, 4School of Medicine, Tufts University, 5Transgenomic Inc., 6Department of Molecular, Cell and Cancer Biology, UMass Medical School

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

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Medicine

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
Rui Liang *1, Wei Dong *1,3, Xiaopeng Shen 1, Xiaoping Peng 1,3, Angie G. Aceves 1,2, Yu Liu 1
1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University

In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation.

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Biology

Pancreatic Islet Embedding for Paraffin Sections
Yahui Kong 1, Pantea Ebrahimpour 1,2, Yu Liu 3, Chaoxing Yang 1, Laura C. Alonso 1
1Diabetes Center of Excellence, UMass Medical School, 2Department of Medicine, Saint Vincent Hospital, 3Department of Pathology, Morphology Core, UMass Medical School

Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.

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Medicine

Traditional Trail Making Test Modified into Brand-new Assessment Tools: Digital and Walking Trail Making Test
Wei Wei *1, Hóngyi Zhào *1,2, Yu Liu 2, Yonghua Huang 1
1Department of Neurology, The Seventh Medical Center of PLA Army General Hospital, 2Department of Neurology, NO 984 Hospital of PLA

Here, we present a protocol to show how to perform two types of cognitive assessment tools derived from the paper-pencil version of the Trail Making Test.

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Medicine

Digital Handwriting Analysis of Characters in Chinese Patients with Mild Cognitive Impairment
Hóngyi Zhào *1,2, Yanhai Zhang *3, Cuiqiao Xia 1, Yu Liu 2, Zhiyi Li 2, Yonghua Huang 1
1Department of Neurology, Chinese PLA General Hospital, 2Department of Neurology, NO 984 Hospital of PLA, 3Department of Cadre Ward, Xijing 986 Hospital, Fourth Military Medical University

This study proposed a digital handwriting analysis of characters in individuals with mild cognitive impairment to find more information than is revealed by traditional pencil–paper handwriting analysis.

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Cancer Research

Characterization and Functional Prediction of Bacteria in Ovarian Tissues
Lanbo Zhao *1, Weichu Zhao *2, Qi Wang *1, Dongxin Liang 1, Yu Liu 3, Guoxing Fu 4, Lu Han 1, Yiran Wang 1, Chao Sun 1, Qing Wang 1, Qing Song 1, Qiling Li 1, Qinrui Lu 1
1Department of Obstetrics and Gynecology, First Affiliated Hospital of Xi’an Jiaotong University, 2School of Social Sciences, University of Manchester, 3Department of Pathology, First Affiliated Hospital of Xi’an Jiaotong University, 4Omega Bioservices Inc

Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).

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Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training
Xin Zhang *1, Cuiqiao Xia *2, Xiuli Zhao 3, Yu Liu 3, Hóngyi Zhào 2,3, Yonghua Huang 2
1Department of Sport, Inner Mongolia Medical University, 2Department of Neurology, The Seventh Medical Center of PLA General Hospital, 3Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.

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