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Pancreatic Islet Embedding for Paraffin Sections

DOI :

10.3791/57931-v

June 29th, 2018

June 29th, 2018

12,132 Views

1Diabetes Center of Excellence, UMass Medical School, 2Department of Medicine, Saint Vincent Hospital, 3Department of Pathology, Morphology Core, UMass Medical School

Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured islets.

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