This work was funded by the Whitehall Foundation and the NIH. We thank Clifford Dax (University of Oregon Technical Support Administration) for his help and expertise in designing a circuit for light delivery.

NOTE: The following protocol is in accordance with the National Institutes of Health guidelines as approved by the University of Oregon Animal Care and Use Committee.
1. Acute Surgery
<ol>
	<li>Anesthetize the animal with a ketamine-medetomidine cocktail, via intraperitoneal (i.p.) injection (Table 1). 
	NOTE: The mice used in these experiments are generated by crossing a cre-dependent ChR2-eYFP transgenic line10 to interneuron driver lines (Pvalb-iCre11, PV+; Sst-iCre12, SOM+; Cr-iCre12, CR+). Viral delivery of ChR2 or related opsins should work equally well, assuming similar expression levels are obtained.</li>
	<li>Before beginning surgery, ensure that the animal exhibits no response to a gentle toe pinch. Re-administer anesthetics throughout the experiment as necessary to maintain this depth of anesthesia. If using injectable anesthetics, optionally implant an i.p. catheter for maintenance injections.</li>
	<li>Keep the animal hydrated with saline or lactated Ringer&#8217;s solution throughout the experiment (approximately 3 ml/kg/hr), for example by using an appropriately diluted anesthetic cocktail for maintenance injections (Table 1).</li>
	<li>Place the animal in a stereotaxic or other head-holding apparatus. Ensure that the skull is well-secured. This is essential for maintaining stable single cell recordings.</li>
	<li>Apply opthalamic ointment to the eyes to prevent dryness. Maintain body temperature at 36.5-37 &#176;C.</li>
	<li>Perform a cisternal drain for additional recording stability. Using a scalpel blade, remove tissue from the posterior face of the skull to expose the cisterna magna. Make a small nick in the dura to drain the cerebrospinal fluid. Use only the very tip of the blade, and avoid contacting the cerebellum or brain stem.</li>
	<li>Using the scalpel, perform a small craniotomy (~2 mm2) over the cortical area of interest (for auditory cortex, roughly -2.3 mm posterior of bregma, 4.5 mm lateral of the midline).</li>
	<li>Remove the dura and cover the exposed cortex with a layer of warm agarose 0.5 - 1 mm thick (1.5% agarose in saline, 0.9% NaCl; apply at ~37 &#176;C). Keep the agarose moist throughout the experiment by periodically applying several drops of saline.</li>
</ol>
2. Recording Set-up
<ol>
	<li>Prepare a tungsten electrode (7-14 M&#937;, 127 &#956;m diameter, 12&#176; tapered tip, epoxy-coated). Superglue the electrode to a glass capillary tube and add heat shrink tubing for grip (Figure 1).</li>
	<li>Mount the electrode on a motorized (or hydraulic) micromanipulator, set to travel orthogonal to the cortical surface. Slide a wire ground under the skin, against the skull. Avoid contact with the muscles on the side of the head and the back of the neck as they can generate electromyographic artifacts.</li>
	<li>Amplify the electrical signal using an extracellular ampli&#64257;er suitable for single-unit recording, preferably equipped with an impedance check mode. Monitor ongoing spiking activity (band pass 300 - 5,000 Hz) with two oscilloscopes and a set of powered speakers. Here, the recorded data is digitized continuously at a sampling rate of 10 kHz; spikes are extracted offline.</li>
	<li>Advance the electrode through the agarose until the tip reaches the surface of the cortex. Observe this step through a microscope. &#8220;Zero&#8221; this position on the micromanipulator.</li>
	<li>To best approximate the depth of recording, visually confirm that the electrode tip exits the cortical surface at a depth of zero when withdrawing the electrode at the end of a penetration. 
	NOTE: A discrepancy between the manipulator reading and the observed electrode position indicates that it has drifted relative to the tissue. This can be caused by instability of the brain or animal, or manipulator drift.
	<ol>
		<li>As an additional check to ensure that this zero set-point corresponds to the surface of the cortex, monitor stimulus-evoked field potentials while advancing through the first several hundred micrometers of tissue. When monitoring field potentials, lower the lower band-pass filter cutoff of the extracellular amplifier to 10 Hz. Confirm that the polarity of the local field potential reverses around a depth of ~100 &#181;m, near the layer I/II border13. This is a reliable reference point for auditory cortex, but it may differ for other cortical areas.</li>
	</ol>
	</li>
	<li>Mount an optical fiber coupled to a blue light source (Figure 2) onto a manual micromanipulator. Using the microscope, position the tip of the fiber as close to the surface of the agarose as possible. Center the beam where the electrode will enter the tissue.</li>
	<li>Regulate the output of the light source with a control unit capable of delivering a TTL (transistor-transistor logic) pulse train of specified width and duration&#8211; e.g., an Arduino (Figure 3), a computer I/O card (as shown), or a commercially available pulse generator.
	<ol>
		<li>Monitor the signal on both oscilloscopes.</li>
	</ol>
	</li>
	<li>Measure total light power (mW) at the tip of the optical fiber using a power meter. Use this value to calculate irradiance (mW/mm2) by dividing by the cross-sectional area of the fiber core. Begin with an intensity value in the range of 10 - 15 mW/mm2 and adjust downward if necessary, until artifacts are eliminated.</li>
	<li>Check for light artifacts with the electrode in the agarose, poised to enter the tissue. Start the search pulse train (e.g., 30 msec light pulses, 500 msec interstimulus interval (ISI)).
	<ol>
		<li>Eliminate transient light artifacts (Figure 4) by repositioning the optical fiber relative to the electrode to change the angle of incident light. If artifacts persist, try decreasing the light power. In the rare case that artifacts cannot be completely eliminated (only minimized), extend the duration of the search pulse. This can aid in identifying true neuronal spikes.</li>
	</ol>
	</li>
</ol>
3. Straight PINP-in&#8217;
<ol>
	<li>Advance the electrode slowly through the brain at a rate of approximately 1 &#181;m/sec. Use one oscilloscope to monitor ongoing activity. On the other, trigger off the laser pulses.</li>
	<li>Listen for the faint &#8216;hash&#8217; of light-evoked spikes on the audio monitor, which indicates that the electrode is approaching a ChR2+ cell and can often be perceived well before the electrical signal is apparent on the oscilloscope. Slow the rate of advance. 
	NOTE: If the cell lies directly in the path of the electrode, the light-evoked activity will grow larger (and louder). As the electrode approaches a single interneuron, the hash will resolve into small but well-defined spikes of uniform size and shape.</li>
	<li>As soon as light-evoked spikes are large enough to trigger off of individually on the oscilloscope, begin doing so. Adjust the scaling on the horizontal axis to observe the exact shape of the spike waveform.</li>
	<li>Stop and wait for the tissue to settle. Resist the temptation to move the electrode closer to the cell. This step is critical for a stable recording. If after several minutes the signal-to-noise ratio has not improved &#8211; that is, the tissue has settled, but it hasn&#39;t brought the cell any closer to the tip of the electrode &#8211; advance 5 &#181;m further and wait again.
	<ol>
		<li>Repeat this process until either the peak or the trough of the action potential can be reliably captured with a voltage threshold set well above the noise floor (e.g., +/-300 &#181;V or greater). 
		NOTE: There&#8217;s little risk of false-positives or ambiguity when PINPing inhibitory interneurons. Most cells can easily follow a train of pulses with duration of 30 msec and inter-pulse interval 500 msec, or faster, with a reliable first spike latency of 2-5 msec (Figure 5). The majority of PV+ cells, in particular, can sustain firing for a full 1 sec (Figure 6). Because these are inhibitory neurons, disynaptic (indirect) activation isn&#8217;t a major concern.</li>
	</ol>
	</li>
	<li>While recording, monitor ongoing activity on the first oscilloscope. Keep a close eye on the size and shape of spikes using the second oscilloscope. Note the quality of their sound on the speaker.
	<ol>
		<li>Listen attentively for abrupt changes, which indicate that the cell is either drawing too close to the electrode (where it risks being impaled or damaged), or is drifting away. If the spikes become large and distorted, back out; if they grow smaller, advance the electrode. Move slowly in 2 &#181;m steps.</li>
		<li>Confirm the quality of the recording post-hoc by superimposing all spike waveforms, aligned to peak or trough. Vary the voltage threshold. Across a wide range of threshold values, there should only ever be one consistent spike shape of uniform height (Figure 5a).</li>
	</ol>
	</li>
	<li>If at any point the signal becomes contaminated with spikes from a neighboring neuron, move on. Advance slowly, on the off-chance that the electrode will pass out of range of the neighboring cell, but remain in range of the target neuron. (This is usually unsuccessful.)</li>
	<li>Move the electrode through the entire depth of cortex (900+ &#181;m) in each penetration. If no light-responsive neurons are encountered after many penetrations or, conversely, recordings are routinely contaminated with spikes from neighboring ChR2- cells, try a new electrode. 
	NOTE: Even within a single batch, the impedance and tip geometry of individual electrodes can vary considerably. Both factors contribute to the effective &#8220;listening radius&#8221; of the electrode, which must be large enough to detect light-evoked spikes while searching, yet restricted enough to enable recording a single interneuron in isolation.</li>
	<li>Test the impedance of electrodes as desired. To avoid damaging the tissue, do this with the tip of the electrode in the upper part of the agarose layer, well outside the brain. Within the range of 7-14 M&#937;, the exact impedance is not a sure predictor of performance, or yield, for this application. That said, it&#8217;s a reasonable proxy for listening radius: lower-impedance electrodes pick up more units; higher-impedance, fewer.</li>
	<li>At the end of the experiment, euthanize the animal by institutionally-approved means, such as anesthetic overdose, or cervical dislocation under a deep plane of anesthesia.</li>
</ol>

<ol>
	<li>Lima, S. Q., Hromadka, T., Znamenskiy, P., Zador, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PINP:+a+new+method+of+tagging+neuronal+populations+for+identification+during+in+vivo+electrophysiological+recording.">PINP: a new method of tagging neuronal populations for identification during in vivo electrophysiological recording.</a> PLoS One. 4, (2009).</li><li>Moore, A. K., Wehr, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parvalbumin-expressing+inhibitory+interneurons+in+auditory+cortex+are+well-tuned+for+frequency.">Parvalbumin-expressing inhibitory interneurons in auditory cortex are well-tuned for frequency.</a> J Neurosci. 33, 13713-13723 (2013).</li><li>Merchant, H., de Lafuente, V., Pena-Ortega, F., Larriva-Sahd, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+impact+of+interneuronal+inhibition+in+the+cerebral+cortex+of+behaving+animals.">Functional impact of interneuronal inhibition in the cerebral cortex of behaving animals.</a> Prog Neurobiol. 99, 163-178 (2012).</li><li>Markram, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interneurons+of+the+neocortical+inhibitory+system.">Interneurons of the neocortical inhibitory system.</a> Nat Rev Neurosci. 5, 793-807 (2004).</li><li>Atallah, B. V., Bruns, W., Carandini, M., Scanziani, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parvalbumin-expressing+interneurons+linearly+transform+cortical+responses+to+visual+stimuli.">Parvalbumin-expressing interneurons linearly transform cortical responses to visual stimuli.</a> Neuron. 73, 159-170 (2012).</li><li>Wilson, N. R., Runyan, C. A., Wang, F. L., Sur, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Division+and+subtraction+by+distinct+cortical+inhibitory+networks+in+vivo.">Division and subtraction by distinct cortical inhibitory networks in vivo.</a> Nature. 488, 343-348 (2012).</li><li>Letzkus, J. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+disinhibitory+microcircuit+for+associative+fear+learning+in+the+auditory+cortex.">A disinhibitory microcircuit for associative fear learning in the auditory cortex.</a> Nature. 480, 331-335 (2011).</li><li>Pi, H. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortical+interneurons+that+specialize+in+disinhibitory+control.">Cortical interneurons that specialize in disinhibitory control.</a> Nature. 503, 521-524 (2013).</li><li>Adesnik, H., Bruns, W., Taniguchi, H., Huang, Z. J., Scanziani, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+neural+circuit+for+spatial+summation+in+visual+cortex.">A neural circuit for spatial summation in visual cortex.</a> Nature. 490, 226-231 (2012).</li><li>Madisen, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+toolbox+of+Cre-dependent+optogenetic+transgenic+mice+for+light-induced+activation+and+silencing.">A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing.</a> Nat Neurosci. 15, 793-802 (2012).</li><li>Hippenmeyer, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+developmental+switch+in+the+response+of+DRG+neurons+to+ETS+transcription+factor+signaling.">A developmental switch in the response of DRG neurons to ETS transcription factor signaling.</a> PLoS Biol. 3, 159 (2005).</li><li>Taniguchi, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+resource+of+Cre+driver+lines+for+genetic+targeting+of+GABAergic+neurons+in+cerebral+cortex.">A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.</a> Neuron. 71, 995-1013 (2011).</li><li>Christianson, G. 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The authors have no competing financial interests.

Although PINP is conceptually straightforward, it can be challenging in practice. A major determinant of success is the choice of electrode. The electrical listening radius is the critical parameter. It must be sufficiently large to detect light-evoked spikes when the tip is still some distance away from a ChR2+ cell, so that one can adjust the rate of advance accordingly. At the same time, it must be restricted enough to enable good single-unit isolation. That is, the electrode must not also pick up spikes from neighbor...

Characterizing the myriad cell types that comprise the mammalian brain has been a central, but long-elusive goal of neurophysiology. For instance, the properties and function of different inhibitory cell types in the cerebral cortex are topics of great interest but are still relatively unknown. This is in part because conventional blind in vivo recording techniques are limited in their ability to distinguish between different cell types. Extracellular spike width can be used to separate putative parvalbumin-positive inhibitory neurons from excitatory pyramidal cells, but this method is subject to both type I and type II errors2,3. Alternatively, recorded neurons can be filled, recovered, and stained to later confirm their morphological and molecular identity, but this is a pain-staking and time-consuming process. Recently, genetically identified populations of inhibitory interneurons have become accessible by means of calcium imaging or visually guided patch recordings. In these approaches, viral or transgenic expression of a calcium reporter (such as GCaMP) or fluorescent protein (such as GFP) allows identification and characterization of cell types defined by promoter expression. These approaches use 2-photon microscopy, which requires expensive equipment, and are also limited to superficial cortical layers due to the light scattering properties of brain tissue. 
Recently, Lima and colleagues1 developed a novel application of optogenetics to target electrophysiological recordings to genetically identified neuronal types in vivo, termed &#8220;PINP&#8221; &#8211; or Photostimulation-assisted Identification of Neuronal Populations. Recordings are performed in mice expressing Channelrhodopsin-2 (ChR2) in specific neuronal subpopulations. Members of the population are identified by their response to a brief flash of blue light. Unlike many other optogenetic applications, the goal is not to manipulate circuit function but simply to identify neurons belonging to a genetically-defined class, which can then be characterized during normal brain function. The technique can be implemented with standard extracellular recording equipment and can therefore serve as an accessible and inexpensive alternative to calcium imaging or visually-guided patching. Here we describe an approach to PINPing specific cell types in the anesthetized auditory cortex, with the expectation that the more general points can be usefully applied in other preparations and brain regions.
In cortex, PINP holds particular promise for investigating the in vivo response properties of inhibitory interneurons. GABAergic interneurons comprise a small, heterogeneous subset of cortical neurons4. Different subtypes, marked by the expression of particular molecular markers, have recently been shown to perform different computational roles in cortical circuits5-9. As genetic tools improve it may eventually be possible to distinguish morphologically- and physiologically-separable types that fall within these broad classes. We here share our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. This strategy was developed specifically for targeting parvalbumin-positive (PV+) cells, but we have found that it works for other interneuron types as well, such as somatostatin-expressing (SOM+) and calretinin-expressing (CR+) interneurons. Although PINPing is conceptually straightforward, it can be surprisingly unyielding in practice. We learned a number of tips and tricks through trial-and-error that may be useful to others attempting the method.

<table border="0" cellpadding="0" cellspacing="0" width="1482">
	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Name of Material/Equipment</td>
			<td style="width:171px;">Company</td>
			<td style="width:341px;">Product/Stock Number</td>
			<td style="width:708px;">Comments/Description</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">ChR2-EYFP Line</td>
			<td style="width:171px;">Jackson Colonies</td>
			<td style="width:341px;">12569</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Pvalb-iCre (PV) Line</td>
			<td style="width:171px;">Jackson Colonies</td>
			<td style="width:341px;">8069</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sst-iCre (SOM) Line</td>
			<td style="width:171px;">Jackson Colonies</td>
			<td style="width:341px;">13044</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cr-iCre (CR) Line</td>
			<td style="width:171px;">Jackson Colonies</td>
			<td>10774</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Agarose</td>
			<td style="width:171px;">Sigma-Aldrich</td>
			<td style="width:341px;">A9793</td>
			<td>Type III-A, High EEO</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Micro Point (dural hook)</td>
			<td style="width:171px;">FST</td>
			<td style="width:341px;">10066-15</td>
			<td style="width:708px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Surgical Scissors</td>
			<td style="width:171px;">FST</td>
			<td>14084-09</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Scalpel</td>
			<td style="width:171px;">FST</td>
			<td>10003-12 (handle), 10011-00 (blades)</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Puralube Ophthalmic Ointment</td>
			<td style="width:171px;">Foster &#38; Smith</td>
			<td style="width:341px;">9N-76855</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Homeothermic Blanket</td>
			<td style="width:171px;">Harvard Apparatus</td>
			<td style="width:341px;"><a name="RANGE!C11">507220F</a></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tungsten Microelectrodes</td>
			<td style="width:171px;">A-M Systems</td>
			<td>577200</td>
			<td>12 M&#937; AC resistance, 127 &#956;m diameter, 12&#176; tapered tip, epoxy-coated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Capillary Glass Tubing</td>
			<td style="width:171px;">Warner Instruments</td>
			<td style="width:341px;">G150TF-3</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Heat Shrink Tubing</td>
			<td style="width:171px;">DigiKey</td>
			<td>A332B-4-ND</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Zapit Accelerator</td>
			<td style="width:171px;">DVA</td>
			<td style="width:341px;">SKU ZA/ZAA</td>
			<td>Use with standard Super Glue.&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Microelectrode AC Amplifier 1800</td>
			<td style="width:171px;">AM Systems</td>
			<td style="width:341px;">700000</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">MP-285 Motorized Micromanipulator</td>
			<td style="width:171px;">Sutter</td>
			<td style="width:341px;">MP-285</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">4-channel Digital Oscilloscopes</td>
			<td style="width:171px;">Tektronix</td>
			<td style="width:341px;">TDS2000C</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Powered Speakers</td>
			<td style="width:171px;">Harman</td>
			<td>Model JBL&#160;Duet</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">Manual Manipulator</td>
			<td style="width:171px;">Scientifica</td>
			<td style="width:341px;">LBM-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">800 &#181;m Fiber Optic Patch Cable</td>
			<td style="width:171px;">ThorLabs</td>
			<td style="width:341px;">FC/PC BFL37-800</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Power Meter</td>
			<td style="width:171px;">ThorLabs</td>
			<td>PM100D (Power Meter), S121C (Standard Power Sensor)</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:263px;">475 nm Cree XLamp XP-E</td>
			<td>DigiKey</td>
			<td>XPEBLU-L1-R250-00Y01DKR-ND</td>
			<td>LED power and efficiency are continually increasing, so we recommend checking for the latest products (www.cree.com).</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Arduino UNO</td>
			<td style="width:171px;">DigiKey</td>
			<td>1050-1024-ND</td>
			<td></td>
		</tr>
	</tbody>
</table>

a guide to in vivo single-unit recording from optogenetically identified cortical inhibitory interneurons

A major challenge in neurophysiology has been to characterize the response properties and function of the numerous inhibitory cell types in the cerebral cortex. We here share our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex using a method developed by Lima and colleagues1. Recordings are performed in mice expressing Channelrhodopsin-2 (ChR2) in specific neuronal subpopulations. Members of the population are identified by their response to a brief flash of blue light. This technique &#8211; termed &#8220;PINP&#8221;, or Photostimulation-assisted Identification of Neuronal Populations &#8211; can be implemented with standard extracellular recording equipment. It can serve as an inexpensive and accessible alternative to calcium imaging or visually-guided patching, for the purpose of targeting extracellular recordings to genetically-identified cells. Here we provide a set of guidelines for optimizing the method in everyday practice. We refined our strategy specifically for targeting parvalbumin-positive (PV+) cells, but have found that it works for other interneuron types as well, such as somatostatin-expressing (SOM+) and calretinin-expressing (CR+) interneurons.

We here share our strategy for obtaining single-unit recordings from genetically-classified inhibitory interneurons in the anesthetized mouse cortex, using an optogenetic method developed by Lima et al.1. Table 1 details the suggested anesthetic cocktail, Ketamine-Medetomidine-Acepromazine (&#8220;KMA&#8221;). Figure 1 depicts a tungsten microelectrode, prepared for recording. Figure 2 contains a circuit diagram for a simple LED control unit. ...

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A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.

A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

A major challenge in neurophysiology has been to characterize the response properties and function of the numerous inhibitory cell types in the cerebral ...

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Research

JoVE Journal

Neuroscience

19.0K Views.  University of Oregon. Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching. 