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University of Texas McGovern Medical School

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Technique to Target Microinjection to the Developing Xenopus Kidney
Bridget D. DeLay 1, Vanja Krneta-Stankic 1,2, Rachel K. Miller 1,2,3,4
1Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, 2Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics, University of Texas MD Anderson Cancer Center

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

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Medicine

Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits
Andrew D. Sligar 1, Gretchen Howe 2, Julia Goldman 3, Patricia Felli 2, Varsha Karanam 1, Richard W. Smalling 2,4, Aaron B. Baker 1,5,6,7
1Department of Biomedical Engineering, University of Texas at Austin, 2Division of Cardiology, University of Texas McGovern Medical School, 3Center for Laboratory Animal Medicine and Care, UT Health Science Center at Houston, 4Memorial Herman Heart and Vascular Center, Texas Medical Center, 5Institute for Cellular and Molecular Biology, University of Texas at Austin, 6The Institute for Computational Engineering and Sciences, University of Texas at Austin, 7Institute for Biomaterials, Drug Delivery and Regenerative Medicine, University of Texas at Austin

We describe a surgical procedure used to induce peripheral ischemia in rabbits with hyperlipidemia and diabetes. This surgery acts as a preclinical model for conditions experienced in peripheral artery disease in patients. Angiography is also described as a means to measure the extent of introduced ischemia and recovery of perfusion.

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