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The Hong Kong Polytechnic University

8 ARTICLES PUBLISHED IN JoVE

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Biology

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
Kenneth K. Kwong 1,2, Lenuta Kloetzer 1,2,3,4, Kelvin K. Wong 5,6, Jia-Qian Ren 1,2, Braden Kuo 1,2,3,4, Yan Jiang 7, Y. Iris Chen 1,2, Suk-Tak Chan 1,2,8, Geoffrey S. Young 9, Stephen T.C. Wong 5,6
1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, 4Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 5Center for biotechnology and Informatics, The Methodist Hospital Research Institute, 6Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, 7Bejing University of Chinese Medicine, 8Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 9Department of Radiology, Brigham and Women's Hospital, Harvard Medical School

Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.

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Genetics

In Vitro Transcription Assays and Their Application in Drug Discovery
Xiao Yang 1, Cong Ma 1,2
1School of Environmental and Life Sciences, University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University

In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

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Medicine

Minimally Invasive Transverse Aortic Constriction in Mice
Aung Moe Zaw 1, Connor M. Williams 2, Helen K. W. Law 3, Billy Kwok Chong Chow 1
1School of Biological Sciences, The University of Hong Kong, 2College of Arts and Science, New York University, 3Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University

Minimally invasive transverse aortic constriction (MTAC) conserves the essentials of regular transverse aortic constriction (TAC) while eliminating the use of a ventilator with tracheal intubation. It proves to be a highly desirable method for high-throughput studies on left ventricular overload, particularly in translational studies.

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Neuroscience

Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
Lin Jiajia *1, Mak Shinghung *2, Zheng Jiacheng 1, Wang Jialing 1, Xu Dilin 1, Hu Shengquan 2, Zhang Zaijun 3, Wang Qinwen 1, Han Yifan 2,4, Cui Wei 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong

This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.

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Biochemistry

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Huang Chunhui 1,2, Xu Dilin 1, Zhang Ke 1, Shentu Jieyi 2, Yan Sicheng 1, Wu Dapeng 3, Wang Qinwen 1, Cui Wei 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, 3School of Materials Science and Chemical Engineering, Ningbo University

This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis.

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Neuroscience

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid
Ling-Yi Liao 1,2,3, Ying-Qian Zhang 4, Benson Wui-Man Lau 5, Qiong Wu 6, Zi-Yan Fan 1,3, Qiang Gao 1,3, Zhi-Hui Zhong 6,7
1Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 2Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, 3Key Laboratory of Rehabilitation Medicine in Sichuan Province, West China Hospital, Sichuan University, 4Department of physiology, Southwest Medical University, 5Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, 6Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 7Sichuan Kangcheng Biotech Co., Inc.

Here, we describe the procedure for a pilot study to explore the effect of repetitive transcranial magnetic stimulation with different frequencies (1 Hz/20 Hz/40 Hz) on Aβ and tau metabolism in rhesus monkey cerebrospinal fluid.

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Medicine

A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry
Jimmy Sung-Hei Tse *1, Ying-Hon Sze *1,2, Jimmy Ka-Wai Cheung 1,2, King-Kit Li 1, Thomas Chuen Lam 1,2,3,4
1Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, 2Centre for Eye and Vision Research (CEVR), 3Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, 4Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University

This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures.

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Neuroscience

Simultaneous Focused Ultrasound Neuromodulation and Fiber Photometry Recording in Free-Moving Mouse
Na Li *1,2, Zihao Chen *1,3, Jiejun Zhu 1, Hanming Zheng 1,4, Jinzhao Xia 1,5, Zhen Yuan 4, Chunlong Fei 2, Lei Sun 3, Zhihai Qiu 1
1Guangdong Institute of Intelligence Science and Technology, 2School of Microelectronics, Xidian University, 3Department of Biomedical Engineering, The Hong Kong Polytechnic University, 4Centre for Cognitive and Brain Sciences and Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau, 5Deep Brain Technology Co., Ltd

The protocol includes transducer manufacturing, parameters reporting, surgical procedure, and signal recording for the entire operational workflow of concurrent focused ultrasound neuromodulation and fiber photometry recording in free-moving mice.

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