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08:57 min
July 27th, 2011
DOI :
10.3791/2836-v
Chapters
0:05
Title
0:56
Preparations for Yeast Transformation
2:19
Yeast Transformation Using a Liquid Handling Robot
6:04
Spotting Assay
7:10
Representative Results: Plasmid Overexpression Screen
8:43
Conclusion
Summary
Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.
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