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Case Western Reserve University

9 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals
Miranda J. Cullins 1, Hillel J. Chiel 1,2,3
1Biology, Case Western Reserve University, 2Neurosciences, Case Western Reserve University, 3Biomedical Engineering, Case Western Reserve University

A technique is described for implanting four in vivo electrodes to monitor the neuromuscular control of feeding behavior in Aplysia californica.

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Immunology and Infection

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
John N. Milligan 1, Emmitt R. Jolly 1
1Department of Biology, Case Western Reserve University

We describe an in vitro method for culturing schistosomula of the flatworm parasite Schistosoma mansoni, via the harvesting and transformation of infective cercariae from the fresh water snail intermediate host Biomphalaria glabrata.

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Medicine

In vivo Dual Substrate Bioluminescent Imaging
Michael K. Wendt 1, Joseph Molter 1, Christopher A. Flask 1, William P. Schiemann 1
1Case Comprehensive Cancer Center, Case Western Reserve University

Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.

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Immunology and Infection

Intravital Imaging of the Mouse Popliteal Lymph Node
H. L. Rachel Liou 1, Jay T. Myers 1, Deborah S. Barkauskas 1, Alex Y. Huang 2
1Department of Pediatrics, Case Western Reserve University , 2Department of Pediatrics, Pathology and Biomedical Engineering, Case Western Reserve University

Recent advances in 2-photon microscopy have enabled real-time in situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.

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Neuroscience

An In Vitro Preparation for Eliciting and Recording Feeding Motor Programs with Physiological Movements in Aplysia californica
Jeffrey M. McManus 1, Hui Lu 1, Hillel J. Chiel 1,2,3
1Department of Biology, Case Western Reserve University , 2Department of Neurosciences, Case Western Reserve University , 3Department of Biomedical Engineering, Case Western Reserve University

We describe a technique to extracellularly record and stimulate from nerves, muscles, and individual identified neurons in vitro while eliciting and observing different types of feeding behaviors in the feeding apparatus of Aplysia.

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Bioengineering

Viral Nanoparticles for In vivo Tumor Imaging
Amy M. Wen 1, Karin L. Lee 1, Ibrahim Yildiz 1, Michael A. Bruckman 1, Sourabh Shukla 1, Nicole F. Steinmetz 2
1Department of Biomedical Engineering, Case Western Reserve University , 2Department of Biomedical Engineering, Radiology, and Materials Science and Engineering, Case Western Reserve University

Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.

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Neuroscience

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
Hui Lu 1, Jeffrey M. McManus 1, Hillel J. Chiel 1,2,3
1Department of Biology, Case Western Reserve University , 2Department of Neurosciences, Case Western Reserve University , 3Department of Biomedical Engineering, Case Western Reserve University

In animals with large identified neurons (e.g. mollusks), analysis of motor pools is done using intracellular techniques1,2,3,4. Recently, we developed a technique to extracellularly stimulate and record individual neurons in Aplysia californica5. We now describe a protocol for using this technique to uniquely identify and characterize motor neurons within a motor pool.

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Medicine

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
Xueming Wu 1, Daniel Lindner 2, Guan-Ping Yu 1, Susann Brady-Kalnay 3, Zheng-Rong Lu 1
1Department of Biomedical Engineering, Case Western Reserve University , 2Department of Translational Hematology & Oncology Research, Cleveland Clinic, Case Western Reserve University , 3Department of Molecular Biology & Microbiology, Case Western Reserve University

To demonstrate MR cancer molecular imaging with a small peptide targeted MRI contrast agent specific to clotted plasma proteins in tumor stroma in a mouse prostate cancer model.

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Neuroscience

Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
Teresa A. Evans 1, Deborah S. Barkauskas 2, Jay T. Myers 3, Alex Y. Huang 3
1Department of Neurosciences, Case Western Reserve University, 2Department of Biomedical Engineering, Case Western Reserve University, 3Department of Pediatrics, Case Western Reserve University

Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.

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