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Hong Kong University of Science and Technology

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Microfluidic Mixers for Studying Protein Folding
Steven A. Waldauer 1, Ling Wu 1, Shuhuai Yao 2, Olgica Bakajin 3, Lisa J. Lapidus 1
1Department of Physics and Astronomy, Michigan State University, 2Department of Mechanical Engineering, Hong Kong University of Science and Technology, 3Center for Biophotonics, University of California, Davis

In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).

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Immunology and Infection

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
Yuanchao Zhan 1, Yaxin Liu 1, Qinglu Zeng 1,2,3
1Department of Ocean Science, Hong Kong University of Science and Technology, 2Division of Life Science, Hong Kong University of Science and Technology, 3HKUST Shenzhen Research Institute

Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.

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Biochemistry

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Alex Y. H. Wong 1, Sheng Xie 1, Ben Zhong Tang 2, Sijie Chen 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.

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Bioengineering

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
Xiufeng Li 1, Lei Kang 1, Claudia T. K. Lo 1, Victor T. C. Tsang 1, Terence T. W. Wong 1
1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology

A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

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Developmental Biology

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
Qihong Zheng *1, Xiaoxiang Xiang *1, Yan Yan 2,3, Huapeng H. Yu 1
1School of Life Sciences, SUSTech University, 2Shenzhen PKU-HKUST Medical Center, 3Division of Life Science, Hong Kong University of Science and Technology

This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.

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