Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

14.2K Views

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06:24 min

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April 21st, 2019

DOI :

10.3791/58669-v

April 21st, 2019

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Alex Y. H. Wong¹, Sheng Xie¹, Ben Zhong Tang², Sijie Chen¹

¹Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, ²Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

Summary

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Fluorescent Silver Staining

Fluorogenic Silver Probe

Protein Staining

Polyacrylamide Gels

Silver Nitrate Staining

Fluorescence Detection

Gel Incubation

Impedance Background Staining

Chapters in this video

0:04

Title

0:36

Preparation of the Gel

1:08

Fixation of the Gel

1:40

Preparation of the AgNO₃ Solution and Silver Impregnation of the Gel

2:41

Fluorogenic Development of the Gel

4:05

Destaining and Imaging

4:34

Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods

5:56

Conclusion

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