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JoVE Journal

Bioengineering

This content is Open Access.

Microfluidic Mixers for Studying Protein Folding

14.9K Views

12:42 min

April 10th, 2012

April 10th, 2012

14,862 Views

0:05

Title

1:05

Fabrication of Microfluidic Mixing Chips

5:46

Mounting and Loading Chips

7:35

Data Collection

9:55

Results: Protein Folding Analysis using Microfluidic Mixers

12:20

Conclusion

In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).

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