Stanford University School of Medicine, Falk Cardiovascular Research Center

7 ARTICLES PUBLISHED IN JoVE

Biology

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model

Tobias Deuse¹, Fumiaki Ikeno², Robert C. Robbins¹, Sonja Schrepfer¹

¹Department of Cardiothoracic Surgery, Stanford University School of Medicine, ²Stanford University School of Medicine

This video demonstrates how to use a preclinical inexpensive and reliable model to study pathobiological and pathophysiological processes of in-stent restenosis development. Longitudinal in vivo monitoring using OCT (Optical Coherence Tomography) and analysis of OCT images are also demonstrated.

Biology

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

Xiaoqin Hua¹, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.

Biology

LAD-Ligation: A Murine Model of Myocardial Infarction

Mandy V.V. Kolk^1,2, Danja Meyberg^1,2, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video demonstrates how to use a fast and reliable model to study pathobiological and pathophysiological processes of myocardial ischemia.

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Mandy Stubbendorff¹, Tobias Deuse^1,2, Anna Hammel¹, Robert C. Robbins², Hermann Reichenspurner¹, Sonja Schrepfer^1,2

¹University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, ²Stanford University School of Medicine

This video demonstrates the orthotopic aortic transplant model as a simple model to study the development of transplant vasculopathy (TVP) in rats.

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Lenard Conradi^1,2, Christiane Pahrmann¹, Stephanie Schmidt¹, Tobias Deuse^1,3, Arne Hansen², Alexandra Eder², Hermann Reichenspurner¹, Robert C. Robbins³, Thomas Eschenhagen², Sonja Schrepfer^1,3

¹Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, ²Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, ³CT Surgery, Stanford University School of Medicine

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Mandy Stubbendorff^*1,2, Xiaoqin Hua^*1,2, Tobias Deuse^1,2,3, Ziad Ali^4,5, Hermann Reichenspurner^2,3, Lars Maegdefessel⁶, Robert C. Robbins⁷, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center, University Hospital Hamburg, ²Cardiovascular Research Center (CVRC) and DZHK University Hamburg, ³Department of Cardiovascular Surgery, University Heart Center Hamburg, ⁴Center for Interventional Vascular Therapy, Division of Cardiology, Columbia University, ⁵Cardiovascular Research Foundation, New York, ⁶Karolinska Institute, Stockholm, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine, Falk Cardiovascular Research Center

This video shows two models of intimal plaque development in murine arteries and emphasizes the differences in myointimal hyperplasia and atherosclerosis.