University of Florida College of Medicine

6 ARTICLES PUBLISHED IN JoVE

Cancer Research

Endobronchial Ultrasound-guided Intratumoral Injection of Cisplatin for the Treatment of Isolated Mediastinal Recurrence of Lung Cancer

Hiren J. Mehta¹, Michael A. Jantz¹

¹Division of Pulmonary/Critical care/Sleep Medicine, University of Florida College of Medicine

The management of isolated recurrent lung cancer in a previously-irradiated field is challenging. Here, we describe an endobronchial ultrasound (EBUS)-guided cisplatin injection for the management of patients with localized lung cancer recurrence in a previously-radiated field.

Cancer Research

Assays for Validating Histone Acetyltransferase Inhibitors

Aaron R. Waddell¹, Daiqing Liao¹

¹Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. However, rigorous methods for validating these inhibitors are needed. Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR.

Neuroscience

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Jose A. Pino^*1, Nora Awadallah^*2, Alessandra M. Norris³, Gonzalo E. Torres²

¹Department of Medicine, School of Medicine, Universidad de Atacama, ²Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, ³Department of Pharmacology and Therapeutics, University of Florida College of Medicine

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Developmental Biology

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Connor D. Johnson¹, Lylybell Y. Zhou¹, Daniel Kopinke^1,2

¹Department of Pharmacology and Therapeutics, University of Florida College of Medicine, ²Myology Institute, University of Florida College of Medicine

The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.

Medicine

Micro-Ultrasound Guided Transperineal Prostate Biopsy: A Clinic-Based Procedure

Rani Ashouri¹, Brianna Nguyen¹, Jeremy Archer², Paul Crispen¹, Padraic O’Malley¹, Li-Ming Su¹, Joseph Grajo^1,3, Sara M. Falzarano⁴, Yahya Acar^5,6,7, David Lizdas^5,6, Samsun Lampotang^1,5,6, Wayne Brisbane^1,6

¹Department of Urology, University of Florida College of Medicine, ²Division of Urology, North Mississippi Medical Center, ³Department of Radiology, University of Florida College of Medicine, ⁴Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, ⁵Department of Anesthesiology, University of Florida College of Medicine, ⁶Center for Safety, Simulation & Advanced Learning Technologies, University of Florida, ⁷Department of Emergency Medicine, Gulhane School of Medicine

This manuscript outlines the steps for performing a micro-ultrasound-guided transperineal prostate biopsy under local anesthesia.

Biology

Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral ß-aminopropionitrile

Jack Bontekoe¹, Gilbert Upchurch², Courtney Morgan¹, Bo Liu³

¹Division of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, ²Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine, ³Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

This protocol describes a methodical surgical approach to modeling advanced abdominal aortic aneurysms in mice by a combination of directly applying elastase to the infrarenal aorta and administering ß-aminopropionitrile through drinking water.