JoVE Logo

Sign In

AOUI Verona

2 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
Gabriella Bergamini *1, Fabio Stellari *2, Angela Sandri *3, Maria M. Lleo 3, Gaetano Donofrio 4, Francesca Ruscitti 5,2, Federico Boschi 6, Andrea Sbarbati 7, Gino Villetti 2, Paola Melotti 8, Claudio Sorio 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona

The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.

image

Medicine

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
George M. Solomon 1, Inez Bronsveld 2, Kathryn Hayes 3, Michael Wilschanski 4, Paola Melotti 5, Steven M. Rowe 1, Isabelle Sermet-Gaudelus 6,7
1Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, 2Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, 3Center for Experimental Medicine, Queens University, Northern Ireland, 4Hadassah Hebrew University Medical Center, Jerusalem, 5Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, 6Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, 7INSERM U 1151, Institut Necker Enfants Malades

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved