National Institute on Aging, National Institutes of Health

5 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Techniques to Induce and Quantify Cellular Senescence

¹Laboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health

Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.

Genetics

Single Molecule Analysis of Laser Localized Psoralen Adducts

Jing Huang¹, Himabindu Gali¹, Julia Gichimu¹, Marina A. Bellani¹, Durga Pokharel¹, Manikandan Paramasivam¹, Michael M. Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.

Cancer Research

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

Jing Huang^*1,2, Peng Guo^*1,2, Marsha A. Moses^1,2

¹Vascular Biology Program, Boston Children's Hospital, ²Department of Surgery, Harvard Medical School and Boston Children's Hospital

Dormant and active cancer cell phenotypes were characterized using quantitative phase imaging. Cell proliferation, migration, and morphology assays were integrated and analyzed in one simple method.

Medicine

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Yuan Yuan¹, Meng-hua Chen¹, Jing Huang², Yuan Tian², Ke Qin³, Zhang Yuan¹, Wen-yan Wang¹, Zhi-jiang Wu¹, Xin-yue Tian¹, Yubin Zhang⁴

¹Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, ²Department of hepatobiliary surgery, The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University, ³Department of transplantation, The Second Affiliated Hospital of Guangxi Medical University, ⁴Digital Technology and Engineering college, Ningbo University of Finance & Economics

This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation.

Neuroscience

Stereotaxic Intracranial Delivery of Chemicals, Proteins or Viral Vectors to Study Parkinson's Disease

Natalie Landeck¹, Melissa Conti Mazza¹, Megan Duffy¹, Christopher Bishop², Caryl E. Sortwell³, Mark R. Cookson¹

¹Cell Biology and Gene Expression Section, National Institute on Aging, National Institutes of Health, ²Behavioral Neuroscience Program, Department of Psychology, Binghamton University, ³Department of Translational Neuroscience, Mercy Health Hauenstein Neuroscience Medical Center, Michigan State University

We describe how to successfully inject solutions into specific brain areas of rodents using a stereotaxic frame. This survival surgery is a well-established method used to mimic various aspects of Parkinson's disease.