Universidad San Sebastián

3 ARTICLES PUBLISHED IN JoVE

Coculture System to Study Osteoblast and Osteoclast Interactions in Prostate Cancer Progression to Hormone Resistance and Metastasis to Bone

Romina F. Amigo^1,2, Macarena A. Fariña¹, Nicolás A. Zuñiga¹, Marcelo A. Villagran³, Eileen M. McNerney¹, Sergio A. Onate¹

¹Molecular Endocrinology and Oncology Laboratory, Departent of Medical Specialties, School of Medicine, Universidad de Concepción, ²School of Health Sciences, Universidad San Sebastián, ³Department of Biochemistry, Universidad Católica de la Santísima Concepción

Prostate cancer bone metastasis is reactivated by fibroblast growth factors and nuclear receptor interactions, together with autocrine/paracrine factors in tumor microenvironment. A coculture cell model using the transwell system of cocultured cells was developed to determine the genes, FGFs and FGF receptors, potentially involved in the progression to bone metastasis.

Immunology and Infection

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Jorge Ibañez-Vega^*1, Danitza Fuentes^*1, Jonathan Lagos^1,2, Jorge Cancino³, María Isabel Yuseff¹

¹Laboratory of Immune Cell Biology, Department of Cellular and Molecular Biology, Pontificia Universidad Católica de Chile, ²Faculty of Medicine, Pontificia Universidad Católica de Chile, ³Centro de Investigaciones en Biología Celular y Biomedicina, Facultad de Ciencia, Universidad San Sebastián

Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.

Neuroscience

Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Daniela Elgueta¹, Ornella Chovar¹, Valentina Ugalde¹, Vanesa Sánchez-Guajardo¹, Alejandra Catenaccio², Felipe Court^2,3,4, Rodrigo Pacheco^1,5

¹Laboratorio de Neuroinmunología, Fundación Ciencia & Vida, ²Center for Integrative Biology, Universidad Mayor, ³FONDAP Geroscience Center for Brain Health and Metabolism, ⁴Buck Institute for Research on Ageing, ⁵Facultad de Medicina y Ciencia, Universidad San Sebastián

This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.