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Janssen Pharmaceutical Companies of Johnson and Johnson

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
Rosa Crespo 1, Wouter Koudstaal 1, Adrian Apetri 1
1Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.

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Neuroscience

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
Donata De Marco 1, Renske Taggenbrock 2,3, Rosa Crespo 3, Wouter Koudstaal 3, Elizabeth Ramsburg 4, Adrian Apetri 3
1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.

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Immunology and Infection

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Stuart T. Perry 1, Elissa Keogh 1, Mike Morton 1, Wouter Koudstaal 2, Gabriel Pascual 1
1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.

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