Bruker Daltonics Inc.

3 ARTICLES PUBLISHED IN JoVE

Environment

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Malak M. Tfaily^*1,2, Rachel M. Wilson^*3, Heather M. Brewer¹, Rosalie K. Chu¹, Heino M. Heyman⁴, David W. Hoyt¹, Jennifer E. Kyle⁵, Samuel O. Purvine¹

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, ²Department of Soil, Water and Environmental Science, University of Arizona, ³Department of Earth Ocean and Atmospheric Sciences, Florida State University, ⁴Bruker Daltonics Inc., ⁵Biological Sciences Division, Pacific Northwest National Laboratory

This protocol describes a single throughput for complementary analytical and omics techniques culminating in a fully-paired characterization of natural organic matter and microbial proteomics in different ecosystems. This approach permits robust comparisons for identifying metabolic pathways and transformations important for describing greenhouse gas production and predicting responses to environmental change.

Chemistry

Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Meiby Fernandez-Rojas¹, Cassandra N. Fuller¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Natarajan V. Bhanu³, Benjamin A. Garcia³, Francisco Fernandez-Lima^1,4

¹Department of Chemistry and Biochemistry, Florida International University, ²Bruker Daltonics Inc., ³Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, ⁴Biomolecular Sciences Institute, Florida International University

An analytical workflow based on liquid chromatography, trapped ion mobility spectrometry, and time-of-flight mass spectrometry (LC-TIMS-ToF MS/MS) for high confidence and highly reproducible "bottom-up" analysis of histone modifications and identification based on principal parameters (retention time [RT], collision cross section [CCS], and accurate mass-to-charge [m/z] ratio).

Chemistry

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega^4,5, Francisco Fernandez-Lima^1,6

¹Deparment of Chemistry and Biochemistry, Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology, Biology Centre CAS, ⁴Department of Biological Sciences, Florida International University, ⁵Department of Parasitology, University of South Bohemia, ⁶Biomolecular Sciences Institute, Florida International University

Presented here is a method for screening lipid structures through stable isotope labeling by using their retention time, mobility, and fragmentation.