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Friedrich-Alexander University Erlangen-Nürnberg

7 ARTICLES PUBLISHED IN JoVE

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Chemistry

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy
Andreas Hutzler *1, Birk Fritsch *1, Michael P. M. Jank 2, Robert Branscheid 3, Erdmann Spiecker 3, Martin März 1,2,4
1Electron Devices (LEB), Department of Electrical, Electronic and Communication Engineering, Friedrich-Alexander University Erlangen-Nürnberg, 2Fraunhofer Institute for Integrated Systems and Device Technology (IISB), 3Institute of Micro- and Nanostructure Research (IMN) and Center for Nanoanalysis and Electron Microscopy (CENEM), Department of Materials Science and Engineering, Friedrich-Alexander University Erlangen-Nürnberg, 4Power Electronics (LEE), Department of Electrical, Electronic and Communication Engineering, Friedrich-Alexander University Erlangen-Nürnberg

A protocol for preparation of graphene-supported microwell liquid cells for in situ electron microscopy of gold nanocrystals from HAuCl4 precursor solution is presented. Furthermore, an analysis routine is presented to quantify observed etching and growth dynamics.

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Biology

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
Dominik J. Fiegle 1, Tilmann Volk 1,2, Thomas Seidel 1,2
1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg

Presented is a protocol for the isolation of human and animal ventricular cardiomyocytes from vibratome-cut myocardial slices. High yields of calcium-tolerant cells (up to 200 cells/mg) can be obtained from small amounts of tissue (<50 mg). The protocol is applicable to myocardium exposed to cold ischemia for up to 36 h.

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Medicine

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model
Decheng Yin *1,2, Jian Fu *1,3, Ida Allabauer 1, Oliver Witzke 4, Song Rong 5, André Hoerning 1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, 5Department of Nephrology, Hannover Medical School

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

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Immunology and Infection

A Modified Surgical Technique for Kidney Transplantation in Mice
Decheng Yin *1,2, Jian Fu *1,3, Rongjun Chen 4, Nelli Shushakova 5, Ida Allabauer 1, Xin-Yi Wei 1, Mario Schiffer 6, Diana Dudziak 7, Song Rong *5, André Hoerning *1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, 5Department of Nephrology, Hannover Medical School, 6Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 7Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

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Engineering

Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators
Jiangao Zhao 1,2, Deming Zhu 2,3, Yuxuan Ma 2,3, Yongling Fu 2,3, Jian Fu 2,3
1Research Institute for Frontier Science, Beihang University, 2School of Mechanical Engineering and Automation, Beihang University, 3Laboratory of Aerospace Servo Actuation and Transmission, Beihang University

We built a simulation model to evaluate pump flow characteristics and performance of the single-shaft coaxial motor-pump assembly in electrohydrostatic actuators and investigate the overall efficiency in a wide set of working conditions of the motor-pump assembly experimentally.

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JoVE Core

A Modeling and Simulation Method for Preliminary Design of an Electro-Variable Displacement Pump
Xu Han 1, Peng Zhang 2, Tatiana Minav 3, Yongling Fu 1, Jian Fu 1
1School of Mechanical Engineering and Automation, Beihang University, 2Beijing Institute of Precision Mechatronics and Controls, 3Innovative Hydraulics and Automation, Tampere University

A simulation model specifically supporting the preliminary design of an electro-variable displacement pump (EVDP) is developed and partially verified by experiments. The control performance, life, reliability, etc., can all be evaluated using the proposed model, which covers the main performance requirements under the EVDP preliminary design task.

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Medicine

Preparation of Human Myocardial Tissue for Long-Term Cultivation
Jules Hamers 1,2, Payel Sen 1,2, Daphne Merkus 1,2,3, Thomas Seidel 4,5, Kun Lu 1,6, Andreas Dendorfer 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.

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