Preparation of Human Myocardial Tissue for Long-Term Cultivation

4.2K Views

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10:58 min

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June 2nd, 2022

DOI :

10.3791/63964-v

June 2nd, 2022

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Jules Hamers¹^,², Payel Sen¹^,², Daphne Merkus¹^,²^,³, Thomas Seidel⁴^,⁵, Kun Lu¹^,⁶, Andreas Dendorfer¹^,²

¹Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, ²German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ³Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, ⁴Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, ⁵Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, ⁶Department of Cardiac Surgery, University Hospital, LMU Munich

Summary

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Human Myocardial Tissue

Long term Cultivation

Ex Vivo Research

Biomimetic Chamber

Contraction Parameters

Cardiac Tissue Slicing

Therapeutic Screening

Cardiotoxic Effects

Isopropanol Solution

Slicing Buffer

Endocardial Trabeculae

Agarose Solidification

Sample Fixation

Chapters in this video

0:04

Introduction

1:01

Preparing Agarose and Vibratome

2:01

Trimming and Embedding the Samples

3:39

Placing the Samples on Cutting Tray and Setting Vibratome

4:17

Medium and Incubator Preparation During Slicing Procedure

5:07

Preparing and Mounting Slices

7:06

Changing Medium

7:51

Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics

10:15

Conclusion

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