Preparation of Human Myocardial Tissue for Long-Term Cultivation
4.4K Views
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10:58 min
•
June 2nd, 2022
DOI :
June 2nd, 2022
•Jules Hamers1,2, Payel Sen1,2, Daphne Merkus1,2,3, Thomas Seidel4,5, Kun Lu1,6, Andreas Dendorfer1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich
Chapters in this video
0:04
Introduction
1:01
Preparing Agarose and Vibratome
2:01
Trimming and Embedding the Samples
3:39
Placing the Samples on Cutting Tray and Setting Vibratome
4:17
Medium and Incubator Preparation During Slicing Procedure
5:07
Preparing and Mounting Slices
7:06
Changing Medium
7:51
Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
10:15
Conclusion
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