University of Campinas

5 ARTICLES PUBLISHED IN JoVE

JoVE Core

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Talita Miguel Marin¹, Nathalia de Carvalho Indolfo⁴, Silvana Aparecida Rocco¹, Murilo de Carvalho^1,2, Marilia Meira Dias¹, Graziele Izalina Vasconcelos Bento¹, Leandro Oliveira Bortot¹, Desirée Cigaran Schuck³, Márcio Lorencini³, Eduardo Pagani¹

¹Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), ²Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), ³Grupo Boticário, ⁴Institute of Biology, University of Campinas

We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.

JoVE Core

Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes

Gabriela F. P. de Souza¹, Airton G. Salles¹

¹Department of Organic Chemistry, Institute of Chemistry, University of Campinas

A persulfate-promoted metal-free benzannulation of α,β-unsaturated compounds and alkynes in water toward the synthesis of unprecedented polyfunctionalized benzenes is reported.

Medicine

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

Rodrigo Menezes Forti^1,2, Marilise Katsurayama^2,3, Giovani Grisotti Martins¹, Lenise Valler^2,3, Andrés Quiroga^1,2, Luiz Simioni¹, Julien Menko⁴, Antonio L. E. Falcão³, Li Min Li^2,5, Rickson C. Mesquita^1,2

¹Institute of Physics, University of Campinas, ²Brazilian Institute of Neuroscience and Neurotechnology, ³Clinical Hospital, University of Campinas, ⁴Department of Emergency Medicine, Albert Einstein College of Medicine, ⁵School of Medical Sciences, University of Campinas

Presented here is a protocol for non-invasively monitoring cerebral hemodynamics of neurocritical patients in real-time and at the bedside using diffuse optics. Specifically, the proposed protocol uses a hybrid diffuse optical systems to detect and display real-time information on cerebral oxygenation, cerebral blood flow and cerebral metabolism.

Neuroscience

Investigation of Activated Mouse Olfactory Sensory Neurons via Combined Immunostaining and in situ Hybridization

Vinicius Miessler de Andrade Carvalho^1,2, Thiago Seike Nakahara^1,2, Fabio Papes¹

¹Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, ²Graduate Program in Genetics and Molecular Biology, Institute of Biology, University of Campinas

This protocol combines in situ hybridization with immunofluorescence to identify olfactory and vomeronasal receptor genes expressed in olfactory sensory neurons after activation by chemical stimuli in the mouse.

Biology

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites

Fernanda Luisa Basei¹, Lívia Alves dos Reis Moura¹, Victor da Cruz Ferreira¹, Andrey Fabricio Ziem Nascimento², Jörg Kobarg¹

¹Faculty of Pharmaceutical Sciences, University of Campinas, ²Brazilian Synchrotron Light Laboratory (LNLS) Brazilian Center for Research in Energy and Materials (CNPEM)

Here, we present a simple and rapid protocol for the detection of protein interaction at DNA damage sites.