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University of Campinas

5 ARTICLES PUBLISHED IN JoVE

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Education

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Talita Miguel Marin 1, Nathalia de Carvalho Indolfo 4, Silvana Aparecida Rocco 1, Murilo de Carvalho 1,2, Marilia Meira Dias 1, Graziele Izalina Vasconcelos Bento 1, Leandro Oliveira Bortot 1, Desirée Cigaran Schuck 3, Márcio Lorencini 3, Eduardo Pagani 1
1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas

We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.

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JoVE Core

Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes
Gabriela F. P. de Souza 1, Airton G. Salles 1
1Department of Organic Chemistry, Institute of Chemistry, University of Campinas

A persulfate-promoted metal-free benzannulation of α,β-unsaturated compounds and alkynes in water toward the synthesis of unprecedented polyfunctionalized benzenes is reported.

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Medicine

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
Rodrigo Menezes Forti 1,2, Marilise Katsurayama 2,3, Giovani Grisotti Martins 1, Lenise Valler 2,3, Andrés Quiroga 1,2, Luiz Simioni 1, Julien Menko 4, Antonio L. E. Falcão 3, Li Min Li 2,5, Rickson C. Mesquita 1,2
1Institute of Physics, University of Campinas, 2Brazilian Institute of Neuroscience and Neurotechnology, 3Clinical Hospital, University of Campinas, 4Department of Emergency Medicine, Albert Einstein College of Medicine, 5School of Medical Sciences, University of Campinas

Presented here is a protocol for non-invasively monitoring cerebral hemodynamics of neurocritical patients in real-time and at the bedside using diffuse optics. Specifically, the proposed protocol uses a hybrid diffuse optical systems to detect and display real-time information on cerebral oxygenation, cerebral blood flow and cerebral metabolism.

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Neuroscience

Investigation of Activated Mouse Olfactory Sensory Neurons via Combined Immunostaining and in situ Hybridization
Vinicius Miessler de Andrade Carvalho 1,2, Thiago Seike Nakahara 1,2, Fabio Papes 1
1Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, 2Graduate Program in Genetics and Molecular Biology, Institute of Biology, University of Campinas

This protocol combines in situ hybridization with immunofluorescence to identify olfactory and vomeronasal receptor genes expressed in olfactory sensory neurons after activation by chemical stimuli in the mouse.

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Biology

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
Fernanda Luisa Basei 1, Lívia Alves dos Reis Moura 1, Victor da Cruz Ferreira 1, Andrey Fabricio Ziem Nascimento 2, Jörg Kobarg 1
1Faculty of Pharmaceutical Sciences, University of Campinas, 2Brazilian Synchrotron Light Laboratory (LNLS) Brazilian Center for Research in Energy and Materials (CNPEM)

Here, we present a simple and rapid protocol for the detection of protein interaction at DNA damage sites.

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