Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Marek J. van Oostrom^*1, Wilke H. M. Meijer^*1, Katharina F. Sonnen¹

¹Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht

A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.