A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing SomitogenesisMarek J. van Oostrom *1, Wilke H. M. Meijer *1, Katharina F. Sonnen 1
1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht
A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.
