Sign In

JoVE Journal

Developmental Biology

This content is Open Access.

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

2.6K Views

08:06 min

March 19th, 2021

March 19th, 2021

2,584 Views

0:04

Introduction

0:50

Generation of the Chip

2:21

Preparing Microfluidics Experiment

3:44

Microfluidics Experiment

5:57

Results: Entrainment Analysis Using Microfluidics Experiments

6:58

Conclusion

Transcript

A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More