Gonçalo Moniz Institute

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Amanda R. Paixão^*1, Beatriz R. S. Dias^*1, Luana C. Palma¹, Natália M. Tavares¹, Cláudia I. Brodskyn¹, Juliana P. B de Menezes¹, Patricia S. T. Veras^1,2

¹Laboratory of Host-Parasite Interaction and Epidemiology, Gonçalo Moniz Institute, ²National Institute of Science and Technology of Tropical Diseases - National Council for Scientific Research and Development (CNPq)

The mechanism associated with phagocytosis in Leishmania infection remains poorly understood. Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells.

Immunology and Infection

Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction

Yasmin da Silva Luz^*1, Amanda R. Paixão^*1, Carla Polyana O. S. Bernardes¹, Thaílla Souza da Silva¹, Natalia Machado Tavares¹, Claudia I. Brodskyn¹, Patricia S. T. Veras¹, Juliana P. B. de Menezes¹

¹Laboratory of Host - Parasite Interaction and Epidemiology, Gonçalo Moniz Institute

Here we study implications of Leishmania-host interaction by exploring Leishmania-infected dendritic cells migration. The differentiation and infection of dendritic cells, migration analysis, and the evaluation of adhesion complexes and actin dynamics are described. This method can be applied to other host cell migration studies when infected with Leishmania or other intracellular parasite species.

Immunology and Infection

In Vitro Differentiation of Human Dendritic Cells and their Markers in Leishmania Infection

Amanda R. Paixão^*1, Rafael Tibúrcio^*1, Elaine Carvalho¹, Thaílla Silva¹, Patrícia S. T. Veras¹, Natalia M. Tavares¹, Juliana P. B. de Menezes¹, Cláudia I. Brodskyn¹

¹Laboratory of Host-Parasite Interaction and Epidemiology, Gonçalo Moniz Institute

Dendritic cells (DCs) are essential components of innate immunity against Leishmania infection. The mechanisms underlying the complex interaction between DCs and Leishmania remain poorly understood. Here, we describe methods to evaluate how Leishmania infection affects the immunobiological function of human DCs, such as migration-related and costimulatory molecule expression.