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hubei university

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Preparation of High-Temperature Sample Grids for Cryo-EM
Yuan-Chih Chang 1,2, Chin-Yu Chen 3,4, Ming-Daw Tsai 1,5
1Institute of Biological Chemistry, Academia Sinica, 2Academia Sinica Cryo-EM Facility, Academia Sinica, 3Department of Life Sciences, National Central University, 4Hubei University, 5Institute of Biochemical Sciences, National Taiwan University

This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM experiments.

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Biology

Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
Meiqi Ma 1, Pei Liu 1, Jingya Yu 2, Runhua Han 3, Letian Xu 1
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, 2Institute of Plant Protection, Wuhan Institute of Landscape Architecture, 3McKetta Department of Chemical Engineering, University of Texas at Austin

To obtain an axenic insect, its egg surface is sterilized, and the hatched larva is subsequently reared using axenic leaves. This method provides an efficient way for axenic insect preparation without administering antibiotics or developing an artificial diet, which can also be applied to other leaf-eating insects.

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Neuroscience

Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network
Shengquan Zhang 1, Xiongxiong Wang 1, Zhihua Liu 1, Shan Jin 1, Chuan-Xi Mao 1
1National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, School of life science, Hubei University

Here, we present a detailed protocol to visualize the microtubule networks in neuromuscular junctions and muscle cells. Combined with the powerful genetic tools of Drosophila melanogaster, this protocol greatly facilitates genetic screening and microtubule-dynamics analysis for the role of microtubule network regulatory proteins in the nervous system.

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