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Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle

2.4K Views

06:56 min

October 8th, 2021

DOI :

10.3791/63195-v

October 8th, 2021


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Axenic Insects

Chapters in this video

0:05

Introduction

0:54

Insect Rearing

1:43

Germ-Free Poplar Culturing

2:40

Egg Surface Sterilization and Rearing Axenic Larvae

3:47

Verification of Axenic Larvae with Culture-Dependent Assays

4:40

Verification of Axenic Individuals with Culture-Independent Assays

5:32

Results: Confirmation of Efficacy of Gut Bacteria Elimination

6:07

Conclusion

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