Yoshiyuki Nishimiya

Assignments of 1H, 13C, and 15N resonances of human lysozyme at 4 degrees C.

Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens.

Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10.

Artificial multimers of the type III antifreeze protein. Effects on thermal hysteresis and ice crystal morphology.

Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner.

A part of ice nucleation protein exhibits the ice-binding ability.

Crystallization and preliminary X-ray crystallographic analysis of Ca2+-independent and Ca2+-dependent species of the type II antifreeze protein.

Activity of a two-domain antifreeze protein is not dependent on linker sequence.

A human antibody fragment with high affinity for biodegradable polymer film.

Effect of annealing time of an ice crystal on the activity of type III antifreeze protein.

Two domains of RD3 antifreeze protein diffuse independently.

Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout.

Crystal structure and mutational analysis of Ca2+-independent type II antifreeze protein from longsnout poacher, Brachyopsis rostratus.

Fully active QAE isoform confers thermal hysteresis activity on a defective SP isoform of type III antifreeze protein.

Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells.

Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis.

Synthetic study and structural analysis of the antifreeze agent xylomannan from Upis ceramboides.

Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice.

NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner.

A simple medium enables bovine embryos to be held for seven days at 4°C.

Antifreeze protein prolongs the life-time of insulinoma cells during hypothermic preservation.