Louise Hetherington

Development of the signalling pathways associated with sperm capacitation during epididymal maturation.

Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation.

Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis.

Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa.

Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa.

The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification.

The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification.

Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation.

Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis.

Label-free quantitation of phosphopeptide changes during rat sperm capacitation.

Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC-MS/MS analysis.

Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation.

Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1.

Head and flagella subcompartmental proteomic analysis of human spermatozoa.

The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition.