Alan W. Flake

In utero hematopoietic stem cell transplantation. A status report.

In utero transplantation for thalassemia.

The current status and future potential of fetal intervention: image is everything.

In utero hematopoietic stem cell transplantation: ontogenic opportunities and biologic barriers.

In utero stem cell transplantation for the treatment of genetic diseases.

The natural history of prenatally diagnosed conjoined twins.

Cystic adenomatoid malformation volume ratio predicts outcome in prenatally diagnosed cystic adenomatoid malformation of the lung.

The EXIT procedure: experience and outcome in 31 cases.

Bone marrow tissue engineering.

Human mesenchymal stem cells: insights from a surrogate in vivo assay system.

Engraftment of bone marrow and fetal liver cells after in utero transplantation in MDX mice.

Influence of congenital heart disease on survival in children with congenital diaphragmatic hernia.

Complete allogeneic hematopoietic chimerism achieved by a combined strategy of in utero hematopoietic stem cell transplantation and postnatal donor lymphocyte infusion.

The ex-utero intrapartum treatment.

High-level allogeneic chimerism achieved by prenatal tolerance induction and postnatal nonmyeloablative bone marrow transplantation.

Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes.

Genetic therapies for the fetus.

AAV-mediated factor IX gene transfer to skeletal muscle in patients with severe hemophilia B.

Management of congenital lung lesions.

Surgery in the human fetus: the future.

Temporary tracheal occlusion in fetal sheep with lung hypoplasia does not improve postnatal lung function.

History of fetal diagnosis and therapy: Children's Hospital of Philadelphia experience.

Mixed chimerism following in utero hematopoietic stem cell transplantation in murine models of hemoglobinopathy.

Congenital high airway obstruction syndrome: natural history and management.

Chorioamniotic membrane separation following open fetal surgery: pregnancy outcome.

Epinephrine inhibits tracheal occlusion induced lung growth in fetal sheep.

Stem cell and genetic therapies for the fetus.

Fetal myelomeningocele repair: short-term clinical outcomes.

Abnormal umbilical cord Dopplers may predict impending demise in fetuses with sacrococcygeal teratoma. A report of 2 cases.

Long-term transgene expression in cardiac and skeletal muscle following fetal administration of adenoviral or adeno-associated viral vectors in mice.

In utero hematopoietic cell transplantation: what are the important questions?

Abnormal umbilical cord Doppler sonograms may predict impending demise in fetuses with sacrococcygeal teratoma. A report of two cases.

The conceptual application of systems theory to stem cell biology: a matter of context.

Complete allogeneic hematopoietic chimerism achieved by in utero hematopoietic cell transplantation and cotransplantation of LLME-treated, MHC-sensitized donor lymphocytes.

Right congenital diaphragmatic hernia: Prenatal assessment and outcome.

Sacrococcygeal teratoma: prenatal assessment, fetal intervention, and outcome.

Mesenchymal stem cells: paradoxes of passaging.

Prenatally diagnosed giant omphaloceles: short- and long-term outcomes.

Thoracoamniotic shunts: fetal treatment of pleural effusions and congenital cystic adenomatoid malformations.

Acute cardiovascular effects of fetal surgery in the human.

Favorable neuroblastoma genes and molecular therapeutics of neuroblastoma.

Heregulin ameliorates the dystrophic phenotype in mdx mice.

Reconstitution of hematopoiesis following intrauterine transplantation of stem cells.

Reproductive outcomes after pregnancy complicated by maternal-fetal surgery.

Transduction of satellite cells after prenatal intramuscular administration of lentiviral vectors.

Surgeons and stem cells: a pragmatic perspective on shifting paradigms.

Optimized large-scale production of high titer lentivirus vector pseudotypes.

In utero stem cell transplantation.

Surfactant protein expression is increased in the ipsilateral but not contralateral lungs of fetal sheep with left-sided diaphragmatic hernia.

Management of fetal mediastinal teratoma.

Impact of continuous intraoperative monitoring on outcomes in open fetal surgery.

The ex utero intrapartum therapy procedure for high-risk fetal lung lesions.

Cystic adenomatoid malformation of the lung: review of genetics, prenatal diagnosis, and in utero treatment.

Severe pulmonary hypoplasia associated with giant cervical teratomas.

Busulfan-conditioned bone marrow transplantation results in high-level allogeneic chimerism in mice made tolerant by in utero hematopoietic cell transplantation.

Tracheal occlusion in fetal rats alters expression of mesenchymal nuclear transcription factors without affecting surfactant protein expression.

Successful revision of portoenterostomy in an infant with biliary atresia.

Prenatal glucocorticoids and exogenous surfactant therapy improve respiratory function in lambs with severe diaphragmatic hernia following fetal tracheal occlusion.

Prenatal transplantation of cytokine-stimulated marrow improves early chimerism in a resistant strain combination but results in poor long-term engraftment.

Structural and functional characterization of bladder smooth muscle in fetal rats with retinoic acid-induced myelomeningocele.

Congenital pulmonary lymphangiectasis sequence: a rare, heterogeneous, and lethal etiology for prenatal pleural effusion.

CD26 inhibition enhances allogeneic donor-cell homing and engraftment after in utero hematopoietic-cell transplantation.

Diagnosis and characterization of fetal sacrococcygeal teratoma with prenatal MRI.

Comparison of outcomes after laparoscopic and open pyloromyotomy at a high-volume pediatric teaching hospital.

Evidence for an immune barrier after in utero hematopoietic-cell transplantation.

Prenatal glucocorticoids improve lung morphology and partially restores surfactant mRNA expression in lambs with diaphragmatic hernia undergoing fetal tracheal occlusion.

Targeted gene transfer to fetal rat lung interstitium by ultrasound-guided intrapulmonary injection.

Gene transfer to ocular stem cells by early gestational intraamniotic injection of lentiviral vector.

Murine bone marrow stromal progenitor cells elicit an in vivo cellular and humoral alloimmune response.

Enhanced epithelial gap closure and increased angiogenesis in wounds of diabetic mice treated with adult murine bone marrow stromal progenitor cells.

Does a myelomeningocele sac compared to no sac result in decreased postnatal leg function following maternal fetal surgery for spina bifida aperta?

Effect of maternal betamethasone administration on prenatal congenital cystic adenomatoid malformation growth and fetal survival.

Persistent expression of hF.IX After tolerance induction by in utero or neonatal administration of AAV-1-F.IX in hemophilia B mice.

The regulatory role of stromal microenvironments in fetal hematopoietic ontogeny.

Postnatal chest wall deformities after fetal thoracoamniotic shunting for congenital cystic adenomatoid malformation.

Liver position and lung-to-head ratio for prediction of extracorporeal membrane oxygenation and survival in isolated left congenital diaphragmatic hernia.

Efficient in vivo targeting of epidermal stem cells by early gestational intraamniotic injection of lentiviral vector driven by the keratin 5 promoter.

Morphologic analysis of the neuromuscular development of the anorectal unit in fetal rats with retinoic acid induced myelomeningocele.

Pulmonary vascular malformations.

OEIS sequence--a rare congenital anomaly with prenatal evaluation and postnatal outcome in six cases.

Emergent excision of a prenatally diagnosed sacrococcygeal teratoma.

Murine bone marrow derived stromal progenitor cells fail to prevent or treat acute graft-versus-host disease.

Outcomes after postnatal resection of prenatally diagnosed asymptomatic cystic lung lesions.

Main bronchus occlusion for treatment of congenital diaphragmatic hernia in fetal lambs.

Prenatal ultrasonographic gastrointestinal abnormalities in fetuses with gastroschisis do not correlate with postnatal outcomes.

Cystic adenomatoid malformations are induced by localized FGF10 overexpression in fetal rat lung.

In utero hematopoietic stem cell transplantation: progress toward clinical application.

Repair of indirect inguinal hernias by "laparoscopic joining of the edges".

Apoptotic cell-mediated immunoregulation of dendritic cells does not require iC3b opsonization.

Prenatal course and postnatal management of peripheral bronchial atresia: association with congenital cystic adenomatoid malformation of the lung.

Fetal tracheal occlusion for severe congenital diaphragmatic hernia in humans: a morphometric study of lung parenchyma and muscularization of pulmonary arterioles.

Correction of ADAMTS13 deficiency by in utero gene transfer of lentiviral vector encoding ADAMTS13 genes.

Intradural inclusion cysts following in utero closure of myelomeningocele: clinical implications and follow-up findings.

Sacrococcygeal teratomas: prenatal surveillance, growth and pregnancy outcome.

Correction of murine ADAMTS13 deficiency by hematopoietic progenitor cell-mediated gene therapy.

Haploidentical in utero hematopoietic cell transplantation improves phenotype and can induce tolerance for postnatal same-donor transplants in the canine leukocyte adhesion deficiency model.

In utero meconium passage in fetuses and newborns with myelomeningocele.

Stem cell and genetic therapies for the fetus.

Prenatal stem cell transplantation and gene therapy.

Maternal alloantibodies induce a postnatal immune response that limits engraftment following in utero hematopoietic cell transplantation in mice.

A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor.

Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling.

Prospective, interdisciplinary follow-up of children with prenatally diagnosed giant omphalocele: short-term neurodevelopmental outcome.

Gastroschisis in mice lacking aortic carboxypeptidase-like protein is associated with a defect in neuromuscular development of the eviscerated intestine.

Reproductive outcomes in subsequent pregnancies after a pregnancy complicated by open maternal-fetal surgery (1996-2007).

Robust in vivo transduction of nervous system and neural stem cells by early gestational intra amniotic gene transfer using lentiviral vector.

Prenatal diagnosis and postnatal resection of intraabdominal enteric duplications.

Predicting the severity of congenital high airway obstruction syndrome.

Neurodevelopmental outcome of infants with congenital diaphragmatic hernia prospectively enrolled in an interdisciplinary follow-up program.

Increased susceptibility to liver damage from pneumoperitoneum in a murine model of biliary atresia.

Fetal surgery: progress and perspectives.

Hepaticoduodenostomy vs hepaticojejunostomy for reconstruction after resection of choledochal cyst.

Amniotic fluid levels of glial fibrillary acidic protein in fetal rats with retinoic acid induced myelomeningocele: a potential marker for spinal cord injury.

Laparoscopic hepaticoduodenostomy versus open hepaticoduodenostomy for reconstruction after resection of choledochal cyst.

Genetic therapy for the fetus: a once in a lifetime opportunity.

Fluoroscopic-assisted endobronchial intubation for single-lung ventilation in infants.

In vivo Differentiation Potential of Mesenchymal Stem Cells: Prenatal and Postnatal Model Systems.

Early delivery as an alternative management strategy for selected high-risk fetal sacrococcygeal teratomas.

Factor IX expression in skeletal muscle of a severe hemophilia B patient 10 years after AAV-mediated gene transfer.

Abnormal brain development and maturation on magnetic resonance imaging in survivors of severe congenital diaphragmatic hernia.

In utero Repair of Myelomeningocele: Rationale, Initial Clinical Experience and a Randomized Controlled Prospective Clinical Trial.

Patch repair for congenital diaphragmatic hernia: is it really a problem?

Early neurodevelopmental outcome of infants with high-risk fetal lung lesions.

Maternal-fetal surgery for structural malformations.

Fetal MRI-calculated total lung volumes in the prediction of short-term outcome in giant omphalocele: preliminary findings.

Use of manipulated stem cells for prenatal therapy.

Animal models for prenatal gene therapy: rodent models for prenatal gene therapy.

Use of magnetic resonance imaging in prenatal prognosis of the fetus with isolated left congenital diaphragmatic hernia.

Ex utero intrapartum treatment in the management of giant cervical teratomas.

Intra-amniotic transient transduction of the periderm with a viral vector encoding TGFβ3 prevents cleft palate in Tgfβ3(-/-) mouse embryos.

A 2-year-old girl with abdominal pain after an action sure to attract attention.

Preschool neurological assessment in congenital diaphragmatic hernia survivors: outcome and perinatal factors associated with neurodevelopmental impairment.

Stem cell and genetic therapies for the fetus.

In utero hematopoietic cell transplantation--recent progress and the potential for clinical application.

Fetal surgical intervention: progress and perspectives.

The effects of fetal surgery on retinopathy of prematurity development.

Perinatal management of congenital oropharyngeal tumors: the ex utero intrapartum treatment (EXIT) approach.

Urologic and anorectal complications of sacrococcygeal teratomas: prenatal and postnatal predictors.

Open fetal surgery for central bronchial atresia.

The contribution of hiatal hernia to severe gastroesophageal reflux disease in patients with gastroschisis.

In utero lung gene transfer using adeno-associated viral and lentiviral vectors in mice.

Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation.

Incidence and factors associated with sensorineural and conductive hearing loss among survivors of congenital diaphragmatic hernia.

In utero stem cell and gene therapy: current status and future perspectives.

The role of echocardiography in the intraoperative management of the fetus undergoing myelomeningocele repair.

Tissue engineering strategies for fetal myelomeningocele repair in animal models.

Fetal myelomeningocele repair: the post-MOMS experience at the Children's Hospital of Philadelphia.

Percutaneous minimal-access fetoscopic surgery for myelomeningocele - not so minimal!

Outcomes of prenatally diagnosed lung lesions in multigestational pregnancies.

Pulmonary hypertension in giant omphalocele infants.

Timing of repair of congenital diaphragmatic hernia in patients supported by extracorporeal membrane oxygenation (ECMO).

Brain-type natriuretic peptide levels correlate with pulmonary hypertension and requirement for extracorporeal membrane oxygenation in congenital diaphragmatic hernia.

Thoracoamniotic shunts for the management of fetal lung lesions and pleural effusions: a single-institution review and predictors of survival in 75 cases.

Prenatal diagnosis of esophageal bronchus--first report of a rare foregut malformation in utero.

Ex utero intrapartum treatment (EXIT) in the management of cervical lymphatic malformation.

Patient characteristics are important determinants of neurodevelopmental outcome during infancy in giant omphalocele.

Consensus statement from the first international conference for in utero stem cell transplantation and gene therapy.

Systemic hypertension in giant omphalocele: An underappreciated association.

Neurodevelopmental outcome at one year of age in congenital diaphragmatic hernia infants not treated with extracorporeal membrane oxygenation.

Immune tolerance induction using fetal directed placental injection in rodent models: a murine model.

Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero.

Axillary lymphatic malformations: Prenatal evaluation and postnatal outcomes.

Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9.

Frequency and complications of inguinal hernia repair in giant omphalocele.

The first 100 infant thoracoscopic lobectomies: Observations through the learning curve and comparison to open lobectomy.

Correction of murine hemoglobinopathies by prenatal tolerance induction and postnatal nonmyeloablative allogeneic BM transplants.

In utero stem cell transplantation and gene therapy: Recent progress and the potential for clinical application.

Experience with Thoracoscopic Repair of Long Gap Esophageal Atresia in Neonates.

Complete tissue coverage achieved by scaffold-based tissue engineering in the fetal sheep model of Myelomeningocele.

Effect of single and multiple courses of maternal betamethasone on prenatal congenital lung lesion growth and fetal survival.

Delayed abdominal closure after congenital diaphragmatic hernia repair.

How to treat a neonate with duodenal atresia and intrapancreatic choledochocele causing persistent hyperbilirubinemia: A case report.

The Intravenous Route of Injection Optimizes Engraftment and Survival in the Murine Model of In Utero Hematopoietic Cell Transplantation.

Younger gestational age is associated with increased risk of adverse neurodevelopmental outcome during infancy in congenital diaphragmatic hernia.

Vector serotype screening for use in ovine perinatal lung gene therapy.

Rate of increase of lung-to-head ratio over the course of gestation is predictive of survival in left-sided congenital diaphragmatic hernia.

Right- versus left-sided congenital diaphragmatic hernia: a comparative outcomes analysis.

Improved pulmonary function in the nitrofen model of congenital diaphragmatic hernia following prenatal maternal dexamethasone and/or sildenafil.

Fetal intrapericardial teratoma: natural history and management including successful in utero surgery.

Neurodevelopmental outcomes at 5years of age in congenital diaphragmatic hernia.

Enhanced in utero allogeneic engraftment in mice after mobilizing fetal HSCs by α4β1/7 inhibition.

Prenatal growth characteristics of lymphatic malformations.

Management and outcomes of scoliosis in children with congenital diaphragmatic hernia.

Induction of Immune Tolerance to Foreign Protein via Adeno-Associated Viral Vector Gene Transfer in Mid-Gestation Fetal Sheep.

Specialty-Based Variation in Applying Maternal-Fetal Surgery Trial Evidence.

Right Congenital Diaphragmatic Hernias: Is There a Correlation between Prenatal Lung Volume and Postnatal Survival, as in Isolated Left Diaphragmatic Hernias?

An extra-uterine system to physiologically support the extreme premature lamb.

Acceptability of In Utero Hematopoietic Cell Transplantation for Sickle Cell Disease.

Responsible surgical innovation and research in maternal-fetal surgery.

An EXTrauterine environment for neonatal development: EXTENDING fetal physiology beyond the womb.

Amniotic Fluid Concentrations of Glial Fibrillary Acidic Protein Do Not Correlate with Prenatal Metrics in Fetuses with Myelomeningocele.

Attitudes of paediatric and obstetric specialists towards prenatal surgery for lethal and non-lethal conditions.

Fetal intervention: Improving evidence and expanding applications.

Growth trajectory and neurodevelopmental outcome in infants with congenital diaphragmatic hernia.

Weighing the Social and Ethical Considerations of Maternal-Fetal Surgery.

Short-Term Neurodevelopmental Outcome in Congenital Diaphragmatic Hernia: The Impact of Extracorporeal Membrane Oxygenation and Timing of Repair.

Rate and Risk Factors Associated with Autism Spectrum Disorder in Congenital Diaphragmatic Hernia.

Safety and efficacy of fetal surgery techniques to close a spina bifida defect in the fetal lamb model: A systematic review.

Umbilical cannulation optimizes circuit flows in premature lambs supported by the EXTra-uterine Environment for Neonatal Development (EXTEND).

Lung function and pulmonary artery blood flow following prenatal maternal retinoic acid and imatinib in the nitrofen model of congenital diaphragmatic hernia.

The influence of gestational age, mode of delivery and abdominal wall closure method on the surgical outcome of neonates with uncomplicated gastroschisis.

Short-Term Neurodevelopmental Outcome in Children Born With High-Risk Congenital Lung Lesions.

Pumpless arteriovenous extracorporeal membrane oxygenation: A novel mode of respiratory support in a lamb model of congenital diaphragmatic hernia.

Complex gastroschisis: Clinical spectrum and neonatal outcomes at a referral center.

Preclinical Canine Model of Graft-versus-Host Disease after In Utero Hematopoietic Cell Transplantation.

Reduced oxygen concentration for the resuscitation of infants with congenital diaphragmatic hernia.

Fetal surgery for spina bifida aperta.

Congenital pulmonary airway malformation: advances and controversies.