quantifying-spatiotemporal-parameters-cellular-exocytosis

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Live imaging of the pHluorin tagged Soluble N-ethylmaleimide-sensitive-factor Attachment protein REceptor (v-SNARE) Vesicle-associated membrane protein 7 ...

Institut Curie, 75005 Paris, France

PSL Research University, Paris, France

Sorbonne Université, Paris, France

TonB-dependent transport is a mechanism for active uptake across the outer membrane of Gram-negative bacteria. The system promotes transport of rare nutrients and was thought to be restricted to iron complexes and vitamin B12. Recent experimental evidence of TonB-energized transport of nickel and different carbohydrates, in addition to bioinformatic-based predictions, challenges this notion and reveals that the number and variety of TonB-dependent substrates is underestimated. It is becoming clear that the chemical nature of the substrates, the energetic requirements for transport and the subsequent translocation across the cytoplasmic membrane can differ from those of the well-studied systems for iron complexes and vitamin B12. These findings question the understanding of TonB-dependent uptake and provide insights into the adaptation of bacteria to their environments.

New substrates for TonB-dependent transport: do we only see the 'tip of the iceberg'?

We developed a computational imaging approach that describes the three-dimensional spatial organization of endomembranes from micromanipulation-normalized mammalian cells with probabilistic density maps. Applied to several well-known marker proteins, this approach revealed the average steady-state organization of early endosomes, multivesicular bodies or lysosomes, endoplasmic reticulum exit sites, the Golgi apparatus and Golgi-derived transport carriers in crossbow-shaped cells. The steady-state organization of each tested endomembranous population was well-defined, unique and in some cases depended on the cellular adhesion geometry. Density maps of all endomembrane populations became stable when pooling several tens of cells only and were reproducible in independent experiments, allowing construction of a standardized cell model. We detected subtle changes in steady-state organization induced by disruption of the cellular cytoskeleton, with statistical significance observed for just 20 cells. Thus, combining micropatterning with construction of endomembrane density maps allows the systematic study of intracellular trafficking determinants.

Probabilistic density maps to study global endomembrane organization.

Many studies have found alterations in the positioning and morphology of intracellular organelles under different experimental conditions. Although the precise quantification of these changes is challenging, it is strongly facilitated in single cells that are seeded on micropatterned substrates. Indeed, the controlled microenvironment of the cell leads to a reproducible distribution of organelles, simplifying image analysis and minimizing the number of cells required for robust phenotypes. Here, we outline how alterations in the intracellular organization of lysosomes and mitochondria, as a result of different growth conditions, can be efficiently quantified in cells seeded on adhesive micropatterns.

Determining the Intracellular Organization of Organelles.

In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of  induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots.  depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of  depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.This article has an associated First Person interview with the first author of the paper.

MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex.

Many studies have investigated the processes that support polarity establishment and maintenance in cells. On the one hand, polarity complexes at the cell cortex and their downstream signaling pathways have been assigned as major regulators of polarity. On the other hand, intracellular organelles and their polarized trafficking routes have emerged as important components of polarity. In this Review, we argue that rather than trying to identify the prime 'culprit', now it is time to consider all these players as a collective. We highlight that understanding the intimate coordination between the polarized cell cortex and the intracellular compass that is defined by organelle positioning is essential to capture the concept of polarity. After briefly reviewing how polarity emerges from a dynamic maintenance of cellular asymmetries, we highlight how intracellular organelles and their associated trafficking routes provide diverse feedback for dynamic cell polarity maintenance. We argue that the asymmetric organelle compass is an indispensable element of the polarity network.

Kristine Schauer

Unité Mixte de Recherche 144 CNRS,
Molecular Mechanisms of Intracellular Transport group,
Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group

Institut Curie, 75005 Paris, France

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Kristine Schauer

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Unité Mixte de Recherche 144 CNRS,Molecular Mechanisms of Intracellular Transport group,Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group

Institut Curie, 75005 Paris, France

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Unité Mixte de Recherche 144 CNRS,
Molecular Mechanisms of Intracellular Transport group,
Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group