conversion-capture-elisa-to-luminex-xmap-assay-using-multiplex

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

The enzyme-linked immunosorbent assay (ELISA) has long been the primary tool for detection of analytes of interest in biological samples for both life ...

intravital-microscopy-imaging-liver-following-leishmania-infection-an

Intravital Microscopy Imaging of the Liver following <em>Leishmania</em> Infection: An Assessment of Hepatic Hemodynamics

Intravital microscopy (IVM) is a powerful optical imaging technique that has made possible the visualization, monitoring and quantification of various ...

Luminex Corporation

University of Aix-Marseille

An experiment evaluated the effect of citrus juice on enhancing serum antioxidant status and on osteoporosis prevention in orchidectomized rats.

Citrus juice modulates bone strength in male senescent rat model of osteoporosis.

Oxidative stress and hypogonadism are two factors linked to the increased incidence of cardiovascular disease in males. Eating fruits and vegetables is known to reduce the incidences of oxidative stress. The objective of this research was to delineate whether drinking daily squeezed orange juice (OJ) or grapefruit juice (GJ) modulates oxidative stress and antioxidant enzymes while impacting cardiovascular risk factors in hypogonad male rats. In the present study, 36 1-year-old male rats were equally divided among the following four treatments: sham (control), orchidectomized (ORX), ORX + OJ, and ORX + GJ. After 60 days of drinking OJ or GJ, antioxidant capacity, cholesterol, and triglycerides in serum and superoxide dismutase (SOD), catalase (CAT), cholesterol, and triglycerides in liver were evaluated. Serum antioxidant capacity and SOD and CAT activities decreased (P < .05), while serum cholesterol and liver triglycerides increased (P < .05) in the ORX group compared with the sham group. In contrast to the ORX group, drinking OJ was ineffective while drinking GJ decreased (P < .05) cholesterol concentration in liver and in serum. Nevertheless, OJ and GJ decreased (P < .05) triglyceride concentration in liver and increased (P < .05) serum antioxidant capacity and SOD and CAT activities compared with the ORX group. In conclusion, drinking OJ or GJ prevented oxidative stress by enhancing total antioxidant capacity and elevating liver antioxidant enzymes while modulating cardiovascular risk factors.

Citrus juice modulates antioxidant enzymes and lipid profiles in orchidectomized rats.

Oxidative stress and hypogonadism are linked to the increased incidence of cardiovascular disease in males. The objective of this research was to delineate whether drinking cranberry juice for 4 months affects antioxidant capacity and lipid profile in orchidectomized rats. Thirty-two 1-year-old male rats were randomized to two groups: a sham-control group (n = 8) and an orchidectomized group (n = 24). The orchidectomized group was divided into three groups of eight and assigned to one of the following treatments: orchidectomy, orchidectomy plus 27% cranberry juice, and orchidectomy plus 45% cranberry juice. At 120 days after initiation of the study, all rats were killed, blood was collected, and plasma was harvested for total antioxidant status, malondialdehyde, nitrate + nitrite, and superoxide dismutase (SOD) activity in liver, and concentrations of cholesterol and triglyceride in liver and in plasma. Orchidectomy depressed (P < .05) plasma antioxidant capacity and SOD activity, elevated (P < .05) nitrate + nitrite and malondialdehyde in plasma, and increased (P < .05) triglyceride and cholesterol values in liver and in plasma. Cranberry juice increased (P < .05) plasma antioxidant capacity and SOD activity and reduced (P < .05) nitrate + nitrite and malondialdehyde concentrations. Drinking cranberry juice did not affect cholesterol concentrations in liver and in plasma. Triglyceride concentration in plasma of orchidectomized rats that were drinking cranberry juice increased (P < .05), but its concentration in liver decreased (P < .05) to the level of shams. The protective effect of cranberry juice from oxidative damage may be mediated by a decrease in nitrate + nitrite and dose-dependent decrease in peroxidation.

Cranberry juice increases antioxidant status without affecting cholesterol homeostasis in orchidectomized rats.

Cervical dislocation is a commonly used method of mouse euthanasia. Euthanasia by isoflurane inhalation is an alternative method which allows the sacrifice of several mice at the same time with an anaesthesia, in the aim to decrease pain and animal distress. The objective of our study was to assess the impact of these two methods of euthanasia on the quality of mouse oocytes. By administering gonadotropins, we induced a superovulation in CD1 female mice. Mice were randomly assigned to euthanasia with cervical dislocation and isoflurane inhalation. Oviducts were collected and excised to retrieve metaphase II oocytes. After microscopic examination, oocytes were classified into three groups: intact, fragmented/cleaved and atretic. Intact metaphase II oocytes were employed for biomedical research. A total of 1442 oocytes in the cervical dislocation group were compared with 1230 oocytes in the isoflurane group. In the cervical dislocation group, 93.1% of the oocytes were intact, versus 65.8% in the isoflurane group (P ≤ 0.001). In light of these results, we conclude that cervical dislocation is the best method of mouse euthanasia for obtaining intact oocytes for biomedical research.

Erica Lopez

Chemistry Research and Development

Luminex Corporation

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics