Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

1.3K Views

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05:49 min

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November 21st, 2023

DOI :

10.3791/65658-v

November 21st, 2023

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Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani¹, Mauro Voi¹, Alexandrina Dabija¹, Rebecca Casula¹, Domenico Luca Minafra¹, Julys da Fonseca Palmeira², Enrique Roberto Argañaraz², Martin Mayora-Neto³, Nigel J. Temperton³, Donato Zipeto¹, Alessandra Ruggiero¹

¹Department of Neuroscience, Biomedicine and Movement, University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway

Transcript

Summary

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Pseudotyped Viruses

Molecular Tool

Humoral Immune Responses

SARS CoV 2

Neutralization Assay

Host virus Interactions

Antibodies

Lentiviral Pseudotypes

Biosafety Level 2

Gene Therapy

HEK293T Cells

Envelope Glycoprotein

Reporter Gene

Infectivity Testing

Relative Luminescence Units

Chapters in this video

0:00

Introduction

1:01

SARS‐CoV‐2 Pseudotype Virus Production and Serum Neutralization Assay

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