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JoVE Journal

Immunology and Infection

This content is Open Access.

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

15.2K Views

08:45 min

March 20th, 2013

March 20th, 2013

15,223 Views

0:05

Title

1:55

Bacterial Strains and Cultures

3:04

Competition Assay

4:21

Qualitative Observation of the Bacterial Killing

6:44

Representative Bacterial Competition Results

7:41

Conclusion

We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.

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