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A Visual Assay to Monitor T6SS-mediated Bacterial Competition

DOI :

10.3791/50103-v

8:45 min

March 20th, 2013

March 20th, 2013

14,816 Views

1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology, Imperial College London

We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.

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T6SS

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