Accedi

Suining Central Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Lijuan Cui¹, Chuan Ye¹, Ting Luo¹, Hongyan Jiang¹, Boan Lai¹, Haojun Wang¹, Zhongyi Chen¹, Yi Li²

¹Pathology Department, Suining Central Hospital, ²General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

The present protocol describesa comprehensive strategy for evaluating the pharmacological action and mechanism of salidroside in inhibiting MCF-7 cell proliferation and migration.

Biology

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Ming Yang^*1,2, Lijuan Cui^*3, Xuesha Liu¹

¹College of Life Sciences, China West Normal University, ²Sichuan Sports College, ³Pathology Department, Suining Central Hospital

Here, we present a protocol to obtain the pVAX1-PRRSV expression vector by introducing suitable restriction sites at the 3' end of the inserts. We can linearize the vector and join DNA fragments to the vector one by one through homologous recombination technology.