Suining Central Hospital

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
Lijuan Cui 1, Chuan Ye 1, Ting Luo 1, Hongyan Jiang 1, Boan Lai 1, Haojun Wang 1, Zhongyi Chen 1, Yi Li 2
1Pathology Department, Suining Central Hospital, 2General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

The present protocol describesa comprehensive strategy for evaluating the pharmacological action and mechanism of salidroside in inhibiting MCF-7 cell proliferation and migration.

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Biology

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
Ming Yang *1,2, Lijuan Cui *3, Xuesha Liu 1
1College of Life Sciences, China West Normal University, 2Sichuan Sports College, 3Pathology Department, Suining Central Hospital

Here, we present a protocol to obtain the pVAX1-PRRSV expression vector by introducing suitable restriction sites at the 3' end of the inserts. We can linearize the vector and join DNA fragments to the vector one by one through homologous recombination technology.

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Biochemistry

Separation and Differential Characterization of Gut Microbial Extracellular Vesicles in Salt-Sensitive Rats under High-Salt Diet Conditions
Lisha Wang *1, Lijuan Cui *2, Luming Qi 3, Youli Tan 4, Shuyu Wang 3, Hongling Liu 1, Changhong Wei 3, Zhangmeng Xu 5, Jie Wang 6
1School of Medical Technology, Chengdu University of Traditional Chinese Medicine, 2Pathology Department, Suining Central Hospital, 3School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, 4Department of Pharmacy, Affiliated Sport Hospital of CDSU, 5Department of Neck, Shoulder, Waist, and Leg Pain, Sichuan Province Orthopedic Hospital, 6Neijiang Hospital of Traditional Chinese Medicine Affiliated to Chengdu University of Traditional Chinese Medicine

The protocol describes the isolation of gut microbial EVs from salt-sensitive rats fed HSD using density gradient centrifugation. EVs were characterized by nanoparticle tracking, TEM, LPS/BCA assays, and 16S rRNA sequencing to analyze the size, morphology, composition, and microbiota origin.