In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer PhotopharmacologyKateryna Horbatok *1,2, Tetyana Makhnii *2, Viktoriia Kosach 2, Volodymyr Danko 1, Andrey Kovalenko 1, Stanislav Fatiushchenkov 1, Petro Borysko 2, Iryna Pishel 2, Oleg Babii 3,4, Anne S. Ulrich 3, Tim Schober 4, Sergii Afonin 3, Igor V. Komarov 1,2,4
1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics
This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo efficacy.
