Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal CellsFangqin Wang 1, Langping Gao 2,3, Xudong Fu 4, Qintao Yan 2, Lidan Hu 1,2, Jianhua Mao 2,3
1National Clinical Research Center for Child Health, The Children's Hospital, Zhejiang University School of Medicine, 2Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 3Zhejiang University School of Medicine, 4Liangzhu Laboratory, Zhejiang University
This protocol describes and compares two representative methods for differentiating hiPSCs into mesenchymal stromal cells (MSCs). The monolayer method is characterized by lower cost, simpler operation, and easier osteogenic differentiation. The embryoid bodies (EBs) method is characterized by lower time consumption.
