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Erasmus University Medical Center - Sophia Children's Hospital

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing
Naomi J. M. Kakiailatu 1, Laura E. Kuil 1,2, Eric Bindels 3, Joke T. M. Zink 3, Michael Vermeulen 3, Veerle Melotte 4, Maria M. Alves 1
1Department of Clinical Genetics, Erasmus University Medical Center - Sophia Children's Hospital, 2Division of Psychosocial Research and Epidemiology, the Netherlands Cancer Institute, 3Department of Hematology, Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center

Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.

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