A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory PlatformTing Zhang 1, Vinitha Dhamotharan 1, Yu Xiao 1,2, Sydne Ballengee 1,3, Jill Pollon 1,4, George K. Gittes 1
1Division of Pediatric Surgery, Department of Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 2North Allegheny High School, 3Wright State University Boonshoft School of Medicine, 4College of Engineering, University of Michigan
As the field of gene therapy continues to evolve, there is a growing need for innovative methods that can address these challenges. Here, a unique method is presented, which streamlines the process of generating high-yield and high-purity AAV vectors using a cell factory platform, meeting the quality standards for in vivo studies.
