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Pushpagiri Institute of Medical Sciences and Research Centre

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Sukumaran Anil 1,2, Nebu G. Thomas 2, Elna P. Chalisserry 2, Yogesh B. Dalvi 2, Ramya Ramadoss 3, Sajith Vellappally 4
1Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, 2Pushpagiri Institute of Medical Sciences and Research Centre, 3Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, 4College of Applied Medical Sciences, King Saud University

This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.

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