Accedi

Oakland University William Beaumont School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Genetics

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Sarah N. Bartolone¹, Maya O. Tree², Michael J. Conway², Michael B. Chancellor^1,3, Laura E. Lamb^1,3

¹Department of Urology, Beaumont Health System, ²Foundational Sciences, College of Medicine, Central Michigan University, ³Oakland University William Beaumont School of Medicine

This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.

Medicine

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases

Khaled Elmasry^1,2,3, Mohamed Moustafa^1,2, Mohamed Al-Shabrawey^1,2

¹Eye Research Center and Department of Foundational Medical Studies, Oakland University William Beaumont School of Medicine, ²Eye Research Institute, Oakland University, ³Human Anatomy and Embryology Department, Mansoura Faculty of Medicine, Mansoura University

This protocol presents and describes steps for the isolation, dissection, culturing, and staining of retinal explants obtained from an adult mouse. This method is beneficial as an ex vivo model for studying different retinal neurovascular diseases such as diabetic retinopathy.