Accedi

Charité-Universitätsmedizin Berlin

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

Pauline Teuffel¹, Miriam Goebel-Stengel^1,2, Tobias Hofmann¹, Philip Prinz¹, Sophie Scharner¹, Jan L. Körner³, Carsten Grötzinger³, Matthias Rose¹, Burghard F. Klapp¹, Andreas Stengel¹

¹Charité Center for Internal Medicine and Dermatology, Division General Internal and Psychosomatic Medicine, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, ²Department of Internal Medicine, Institute of Neurogastroenterology and Motility, Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin, ³Department of Hepatology and Gastroenterology, Molecular Cancer Research Center (MKFZ), Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin

The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.

Biology

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Christopher Weidner¹, Matthias Steinfath¹, Elisa Wistorf¹, Michael Oelgeschläger¹, Marlon R. Schneider¹, Gilbert Schönfelder^1,2

¹Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), ²Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin

We provide a standardized protocol for the use of gene set enrichment analysis of transcriptomic data to identify an ideal mouse model for translational research.
This protocol can be used with DNA microarray and RNA sequencing data and can further be extended to other omics data if data are available.