Charité-Universitätsmedizin Berlin
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
Pauline Teuffel 1, Miriam Goebel-Stengel 1,2, Tobias Hofmann 1, Philip Prinz 1, Sophie Scharner 1, Jan L. Körner 3, Carsten Grötzinger 3, Matthias Rose 1, Burghard F. Klapp 1, Andreas Stengel 1
1Charité Center for Internal Medicine and Dermatology, Division General Internal and Psychosomatic Medicine, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, 2Department of Internal Medicine, Institute of Neurogastroenterology and Motility, Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin, 3Department of Hepatology and Gastroenterology, Molecular Cancer Research Center (MKFZ), Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin
The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.
Biology
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research
Christopher Weidner 1, Matthias Steinfath 1, Elisa Wistorf 1, Michael Oelgeschläger 1, Marlon R. Schneider 1, Gilbert Schönfelder 1,2
1Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin
We provide a standardized protocol for the use of gene set enrichment analysis of transcriptomic data to identify an ideal mouse model for translational research.
This protocol can be used with DNA microarray and RNA sequencing data and can further be extended to other omics data if data are available.
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