Accedi

Integrated Dna Technologies, Inc.

2 ARTICLES PUBLISHED IN JoVE

Biology

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Yang Song^*1, Xuemei Zhang^*1, Lingyan Huang², Mark A. Behlke², Andrew Tsourkas¹

¹Department of Bioengineering, University of Pennsylvania, ²Integrated DNA Technologies, Inc.

Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.

Neuroscience

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Irina Sinakevitch^1,2, Zev Kurtzman^*2, Hyun G. Choi^*2, David Arturo Ruiz Pardo³, Romain A. Dahan², Nathaniel Klein¹, Branimir Bugarija⁴, Erik Wendlandt⁴, Brian H. Smith²

¹Department of Neuroscience, University of Arizona, ²School of Life Sciences, Arizona State University, ³Department of Scientific and Technologic Investigations, University of Sonora, ⁴Integrated DNA Technologies, Inc.

Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.