Expansion of Human Peripheral Blood γδ T Cells using ZoledronateMakoto Kondo 1,2, Takamichi Izumi 1,2, Nao Fujieda 1,2, Atsushi Kondo 1,2, Takeharu Morishita 1,2, Hirokazu Matsushita 1, Kazuhiro Kakimi 1
1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd
A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.
