Accedi

beijing institute of technology

5 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
William C.W. Chen 1, Arman Saparov 2,3, Mirko Corselli 4, Mihaela Crisan 5, Bo Zheng 6, Bruno Péault 7,8, Johnny Huard 9
1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Pittsburgh, 3Nazarbayev University Research and Innovation System, Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 5Department of Cell Biology, Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine, Oregon Health & Science University, 7Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, 8David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 9Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh

Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.

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Engineering

New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
Xu Yan *1,3,4,5, Zhaozhong Meng *2, Jiting Ouyang 2, Yajun Qiao 2, Fang Yuan 1,3,4,5
1Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, 2School of Physics, Beijing Institute of Technology, 3Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, 4Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, 5China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University

A protocol for the neuroprotective application of low-dose atmospheric pressure plasma treatment on glucose deprivation-induced SH-SY5Y injuries.

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Neuroscience

Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression
Weipeng Zheng *1, Xiuhang Ruan *2, Xinhua Wei 3, Fangtian Xu 4, Yuanping Huang 1, Ning Wang 1, Haoyi Chen 5, YingJie Liang 6, Wende Xiao 6, Xin Jiang 1, Shifeng Wen 6
1Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, 2Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, 3Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, 4Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, 5Department of Orthopedics, Guangzhou Chest Hospital, 6Department of Orthopedics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou Medical University

Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression.

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Biochemistry

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
Yi-Xin Huo 1,2, Bo Zheng 1, Ning Wang 1, Yunpeng Yang 1, Xinxin Liang 1, Xiaoyan Ma 1
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, 2UCLA Institute for Technology Advancement (Suzhou)

This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.

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Behavior

Tactile Semiautomatic Passive-Finger Angle Stimulator (TSPAS)
Wu Wang 1, Jiajia Yang 2,4, Yinghua Yu 2,3,4, Qiong Wu 5,2, Satoshi Takahashi 2, Yoshimichi Ejima 2, Jinglong Wu 6,2
1Cognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama University, 2Cognitive Neuroscience Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3Center for Information and Neural Networks, National Institute of Information and Communications Technology, 4Section on Functional Imaging Methods, National Institute of Mental Health, 5School of Education, Suzhou University of Science and Technology, 6Beijing Institute of Technology

Presented is the tactile semiautomated passive-finger angle stimulator TSPAS, a new way to assess tactile spatial acuity and tactile angle discrimination using a computer-controlled tactile stimulus system that applies raised angle stimuli to a subject's passive fingerpad, while controlling for movement speed, distance, and contact duration.

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