Accedi

beijing institute of technology

5 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

William C.W. Chen¹, Arman Saparov^2,3, Mirko Corselli⁴, Mihaela Crisan⁵, Bo Zheng⁶, Bruno Péault^7,8, Johnny Huard⁹

¹Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, ²Department of Orthopedic Surgery, University of Pittsburgh, ³Nazarbayev University Research and Innovation System, Nazarbayev University, ⁴Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, ⁵Department of Cell Biology, Erasmus MC Stem Cell Institute, ⁶OHSU Center for Regenerative Medicine, Oregon Health & Science University, ⁷Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, ⁸David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, ⁹Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh

Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.

Engineering

New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells

Xu Yan^*1,3,4,5, Zhaozhong Meng^*2, Jiting Ouyang², Yajun Qiao², Fang Yuan^1,3,4,5

¹Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, ²School of Physics, Beijing Institute of Technology, ³Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, ⁴Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing Tiantan Hospital, Capital Medical University, ⁵China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University

A protocol for the neuroprotective application of low-dose atmospheric pressure plasma treatment on glucose deprivation-induced SH-SY5Y injuries.

Neuroscience

Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression

Weipeng Zheng^*1, Xiuhang Ruan^*2, Xinhua Wei³, Fangtian Xu⁴, Yuanping Huang¹, Ning Wang¹, Haoyi Chen⁵, YingJie Liang⁶, Wende Xiao⁶, Xin Jiang¹, Shifeng Wen⁶

¹Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, ²Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, ³Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, ⁴Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, ⁵Department of Orthopedics, Guangzhou Chest Hospital, ⁶Department of Orthopedics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou Medical University

Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord compression.

Biochemistry

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Yi-Xin Huo^1,2, Bo Zheng¹, Ning Wang¹, Yunpeng Yang¹, Xinxin Liang¹, Xiaoyan Ma¹

¹Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, ²UCLA Institute for Technology Advancement (Suzhou)

This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.

Behavior

Tactile Semiautomatic Passive-Finger Angle Stimulator (TSPAS)

Wu Wang¹, Jiajia Yang^2,4, Yinghua Yu^2,3,4, Qiong Wu^5,2, Satoshi Takahashi², Yoshimichi Ejima², Jinglong Wu^6,2

¹Cognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama University, ²Cognitive Neuroscience Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, ³Center for Information and Neural Networks, National Institute of Information and Communications Technology, ⁴Section on Functional Imaging Methods, National Institute of Mental Health, ⁵School of Education, Suzhou University of Science and Technology, ⁶Beijing Institute of Technology

Presented is the tactile semiautomated passive-finger angle stimulator TSPAS, a new way to assess tactile spatial acuity and tactile angle discrimination using a computer-controlled tactile stimulus system that applies raised angle stimuli to a subject's passive fingerpad, while controlling for movement speed, distance, and contact duration.