Accedi

School of Medical Sciences, University of New South Wales

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Production and Titering of Recombinant Adeno-associated Viral Vectors

Christina McClure^*1, Katy L. H. Cole^*1, Peer Wulff¹, Matthias Klugmann², Andrew J. Murray^1,3

¹School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, ²Translational Neuroscience Facility and Department of Physiology, School of Medical Sciences, University of New South Wales, ³Department of Biochemistry and Molecular Biophysics, Columbia University

Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.