Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial MembranesJennifer Walshe 1, Najla Al Khaled Abdulsalam 2, Shuko Suzuki 1, Traian V. Chirila 1,3,4,5,6,7,8, Damien G. Harkin 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia
This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.
