Accedi

Denver VA Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Ron Bouchard^1,2, Thomas Chong^1,2, Subbiah Pugazhenthi^1,2

¹Section of Endocrinology, Denver VA Medical Center, ²Department of Medicine, University of Colorado Denver School of Medicine

Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.

Medicine

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

Marilee J. Wick^1,2, Julie W. Harral^1,2, Zoe L. Loomis^1,2, Edward C. Dempsey^1,2,3

¹Cardiovascular Pulmonary Research Laboratory, University of Colorado Denver, ²Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, ³Denver VA Medical Center

In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues.